Near-field scanning optical microscopy of single molecules by femtosecond two-photon excitation
| Autor: | Michael K. Lewis, Ari Gafni, Peter Wolanin, Duncan G. Steel |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Materials science
Super-resolution microscopy business.industry Scanning confocal electron microscopy Physics::Optics Laser Atomic and Molecular Physics and Optics law.invention Optics Two-photon excitation microscopy law Light sheet fluorescence microscopy Microscopy Near-field scanning optical microscope business Vibrational analysis with scanning probe microscopy |
| Zdroj: | Optics letters. 23(14) |
| ISSN: | 0146-9592 |
| Popis: | We present a demonstration of near-field scanning optical microscopy of single molecules based on ultrafast two-photon-induced fluorescence. Measurements were performed by use of 100-fs pulses at 800 nm from a Ti:sapphire laser to excite the two-photon transition in Rhodamine B molecules. Although near-field probes are normally metal coated to achieve superresolution, we used uncoated tips to achieve sufficiently high optical powers to generate acceptable fluorescence emission rates. Images of single molecules demonstrate a resolution of ~175nm(< lambda/4) on a topographically smooth surface, which surpasses the apparent lambda/2 resolution limit for uncoated tips operating in the linear response regime. |
| Databáze: | OpenAIRE |
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