High responsiveness of HLA-B57-restricted Gag-specific CD8(+) T cells in vitro may contribute to the protective effect of HLA-B57 in HIV-infection
| Autor: | Erwan Piriou, Christine A. Jansen, Frank Miedema, Nening M. Nanlohy, Debbie van Baarle, Erik H Manting, Iris M. De Cuyper, Stefan Kostense, Kristin Vandenberghe |
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| Přispěvatelé: | Landsteiner Laboratory |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Pore Forming Cytotoxic Proteins
HIV Antigens T cell Immunology Gene Products gag HIV Infections CD8-Positive T-Lymphocytes In Vitro Techniques Biology Granzymes CCL5 HLA-B8 Antigen Epitopes Interferon-gamma Interleukin 21 HLA-A2 Antigen medicine Humans Immunology and Allergy Cytotoxic T cell Amino Acid Sequence Antigen-presenting cell Membrane Glycoproteins Base Sequence Perforin Serine Endopeptidases Natural killer T cell Molecular biology Tumor Necrosis Factor Receptor Superfamily Member 7 medicine.anatomical_structure HLA-B Antigens DNA Viral Mutation HIV-1 Interleukin 12 CD8 |
| Zdroj: | European journal of immunology, 35(1), 150-158. Wiley-VCH Verlag European Journal of Immunology, 35(1), 150. Wiley-VCH Verlag |
| ISSN: | 0014-2980 |
| DOI: | 10.1002/eji.200425487 |
| Popis: | HLA-B57 has been shown to be associated with long-term asymptomatic HIV-1 infection. To investigate the biological mechanism by which the HLA-B57 allele could protect from HIV-1 disease, we studied both the number of CD8(+) T cells as well as CD8(+) T cell responsiveness directed to different HIV-1 Gag peptides presented by HLA-A2, -B8 or -B57. T cells specific for the HLA-B57 peptide KAFSPEVIPMF responded more readily and to a higher extend to antigenic stimulation in vitro than T cells specific for the HLA-A2 peptide SLYNTVATL or the HLA-B8 peptide EIYKRWII. This phenomenon was reproducible with T cells from individuals expressing HLA-B57 in combination with one or both of the other alleles and was persistent during long-term follow-up. Lower reactivity of A2- and B8-restricted T cells was not explained by mutations in the B8- or A2-restricted Gag-peptides. Moreover, no correlation between peptide mutation frequency and IFN-gamma production by the corresponding Gag-specific T cells was observed. In conclusion, functional differences were observed between T cells specific for HIV epitopes derived from the same protein presented by different HLA molecules. B57-restricted KAFSPEVIPMF-specific CD8(+) T cells have relatively high responsiveness, which could contribute to the protective effect of HLA-B57 in HIV infection. |
| Databáze: | OpenAIRE |
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