miRNA Profiling: How to Bypass the Current Difficulties in the Diagnosis and Treatment of Sarcomas
| Autor: | Laurent Alberti, Jean-Yves Blay, Anne-Valérie Decouvelaere, Anthony Besse, Dominique Ranchère-Vince, Angélique Gougelet, Adeline Duc, Daniel Pissaloux, Jennifer Perez |
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| Přispěvatelé: | Oncogénèse et progression tumorale, Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Conticanet Consortium (FP6-06188), IID-Biotech, Département d'anatomopathologie, biopathologie, Centre Léon Bérard [Lyon], European Organisation for Research and Treatment of Cancer [Bruxelles] (EORTC), European Cancer Organisation [Bruxelles] (ECCO), GOUGELET, Angélique, Service d'Oncologie Médicale, Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
musculoskeletal diseases
Pathology medicine.medical_specialty Article Subject medicine.medical_treatment [SDV]Life Sciences [q-bio] Cell [SDV.CAN]Life Sciences [q-bio]/Cancer lcsh:RC254-282 03 medical and health sciences 0302 clinical medicine Complex Karyotype microRNA TaqMan Medicine Radiology Nuclear Medicine and imaging Rhabdomyosarcoma 030304 developmental biology 0303 health sciences Chemotherapy business.industry medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens 3. Good health [SDV] Life Sciences [q-bio] medicine.anatomical_structure Oncology Cell culture 030220 oncology & carcinogenesis Cancer research Osteosarcoma business Research Article |
| Zdroj: | Sarcoma, Vol 2011 (2011) Sarcoma Sarcoma, Hindawi Publishing Corporation, 2011, 2011, pp.460650. ⟨10.1155/2011/460650⟩ BASE-Bielefeld Academic Search Engine |
| ISSN: | 1369-1643 1357-714X |
| Popis: | International audience; Sarcomas are divided into a group with specific alterations and a second presenting a complex karyotype, sometimes difficult to diagnose or with few therapeutic options available. We assessed if miRNA profiling by TaqMan low density arrays could predict the response of undifferentiated rhabdomyosarcoma (RMS) and osteosarcoma to treatment. We showed that miRNA signatures in response to a therapeutic agent (chemotherapy or the mTOR inhibitor RAD-001) were cell and drug specific on cell lines and a rat osteosarcoma model. This miRNA signature was related to cell or tumour sensitivity to this treatment and might be not due to chromosomal aberrations, as revealed by a CGH array analysis of rat tumours. Strikingly, miRNA profiling gave promising results for patient rhabdomyosarcoma, discriminating all types of RMS: (Pax+) or undifferentiated alveolar RMS as well as embryonal RMS. As highlighted by these results, miRNA profiling emerges as a potent molecular diagnostic tool for complex karyotype sarcomas. |
| Databáze: | OpenAIRE |
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