Preparation of biocompatible and biodegradable silk fibroin/chitin/silver nanoparticles 3D scaffolds as a bandage for antimicrobial wound dressing
| Autor: | Mahdi Rahimi, Hossein Samadi Kafil, Ramin Karimian, Mojtaba Ghanbari Mehrabani, Bahareh Mehramouz |
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| Rok vydání: | 2018 |
| Předmět: |
Staphylococcus aureus
Silver Biocompatibility Metal Nanoparticles Fibroin Chitin 02 engineering and technology 010402 general chemistry 01 natural sciences Biochemistry Silver nanoparticle Cell Line Nanocomposites chemistry.chemical_compound Wound care Anti-Infective Agents Structural Biology Candida albicans Materials Testing Escherichia coli Humans Molecular Biology Nanocomposite integumentary system General Medicine 021001 nanoscience & nanotechnology Antimicrobial Bandages 0104 chemical sciences chemistry Fibroins 0210 nano-technology Wound healing Biomedical engineering |
| Zdroj: | International Journal of Biological Macromolecules. 114:961-971 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2018.03.128 |
| Popis: | One of the most serious challenge in wound care is difficulty of the infection control in wound healing process. Wound dressings with antimicrobial effects are useful to minimize the bacterial infections of wounds. In this study a biocompatible and biodegradable silk fibroin/chitin nanocomposite scaffolds with various content of silver nanoparticles (0.001, 0.01, and 0.1%) were fabricated via freeze-drying method for successful wound dressing application. The nanocomposite scaffolds have biocompatibility with high antimicrobial effects, good mechanical properties, and high porosity. Furthermore, swelling and water uptake, blood clotting, and biodegradability were also investigated. The antimicrobial evaluation with both disc diffusion method and colony counts imply that nanocomposite scaffolds have high antimicrobial activity and could successfully inhibit the growth of E. coli, S. aureus, and C. albicans. Also proliferation, cell viability, and cell attachment with MTT assay and DAPI staining on nHFFF2 cell, have proved the cytocompatibility nature of the nanocomposite scaffolds. All results revealed that prepared nanocomposite scaffolds are good candidate for wound dressing and could be use in further in-vivo uses. |
| Databáze: | OpenAIRE |
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