Immune responses against autologous tumor and human papilloma virus in lymph nodes from patients with penile cancer
| Autor: | Ola Winqvist, Mats Hedlund, Katrine Riklund, Ali Zirakzadeh, Lu Zhang, Ping Sheng Hu, Amir Sherif, Jesper Rosvall |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male Urological Oncology 030232 urology & nephrology CD8-Positive T-Lymphocytes Lymphocyte Activation lcsh:RC870-923 lymph node excision 0302 clinical medicine Human Papillomavirus Recombinant Vaccine Quadrivalent Types 6 11 16 18 penile neoplasms Urologi och njurmedicin Papillomaviridae T-lymphocytes Aged 80 and over biology Middle Aged Tumor antigen medicine.anatomical_structure 030220 oncology & carcinogenesis Original Article adoptive immunity medicine.drug Urology T cell Penile Neoplasm Immunophenotyping Adoptive immunity 03 medical and health sciences Viral Proteins Immune system Antigen Antigens Neoplasm medicine Penile cancer Humans Urology and Nephrology t-lymphocytes Aged papillomaviridae business.industry Gardasil medicine.disease biology.organism_classification lcsh:Diseases of the genitourinary system. Urology Penile neoplasms Lymph node excision Cancer research Lymph Nodes business |
| Zdroj: | Investigative and Clinical Urology, Vol 62, Iss 1, Pp 39-46 (2021) Investigative and Clinical Urology |
| Popis: | PURPOSE: Nearly half of penile cancers are related to human papillomavirus (HPV) infection. Investigations of tumor- and HPV-specific T cell reactivity in regional lymph nodes (LNs) from patients with penile cancer are warranted. MATERIALS AND METHODS: In this study, single-cell suspensions from LNs and peripheral blood from 11 patients with penile cancer were stained with antibodies for lymphocyte markers and analyzed by fluorescence-activated cell sorting (FACS). DNA was extracted from the tumor tissue and HPV status was investigated by PCR. RESULTS: T-cell reactivity against autologous tumor-extract and against the HPV-vaccine Gardasil® was tested by flow-cytometric assay of specific cell-mediated immune response in activated whole blood (FASCIA). CD4+/CD8+ ratios were significantly lower in HPV positive LNs (p |
| Databáze: | OpenAIRE |
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