Comparison between collision induced dissociation of electrosprayed protonated peptides in the up-front source region and in a low-energy collision cell
| Autor: | J. I. T. van Wijk, Johan Haverkamp, W. Heerma, W. D. van Dongen, B. N. Green |
|---|---|
| Přispěvatelé: | Centraal Instituut voor Voedingsonderzoek TNO |
| Rok vydání: | 1999 |
| Předmět: |
Collision-induced dissociation
Analytical chemistry Protonation Mass spectrometry Spectral line Analytical Chemistry Ion Nuclear Experiment Instrumentation Spectroscopy Nutrition Quantitative Biology::Biomolecules Tandem Chemistry digestive oral and skin physiology Organic Chemistry Methodology Peptide fragment Peptide Fragments Peptide Quadrupole Mass spectrum Comparative study Peptides |
| Zdroj: | Rapid Communications in Mass Spectrometry, 17, 13, 1712-1716 |
| ISSN: | 1097-0231 0951-4198 |
| DOI: | 10.1002/(sici)1097-0231(19990915)13:17<1712::aid-rcm703>3.0.co;2-8 |
| Popis: | A systematic comparison between the up-front Collision induced dissociation (CID) mass spectra and low-energy CID tandem mass spectra from twenty-one singly and/or doubly charged peptides has been made. CID spectra of the peptides were recorded at different electrode voltages in the up-front source region of a single quadrupole instrument and different collision energies in the collision cell of a tandem quadrupole instrument. It was observed that up-front CID and low-energy CID yielded comparable product ion spectra from protonated peptides, and that the instrumental settings necessary for obtaining comparable CID mass spectra from the two methods are correlated.Chemicals/CAS: Peptide Fragments; Peptides |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text