Cow blood - A superior storage option in forensics?
| Autor: | Ursula Windberger, Andreas Sparer, Johann Huber |
|---|---|
| Přispěvatelé: | Windberger, Ursula |
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: |
[SDV] Life Sciences [q-bio]
Erythrocyte-Membrane Oligomeric State Bank Storage Band-3 Viscosity Stability Protein History Multidisciplinary Polymers and Plastics drip pattern hematology blood storage Cow blood ageing rheology Business and International Management bloodstain pattern analysis Industrial and Manufacturing Engineering |
| DOI: | 10.1016/j.heliyon.2023.e14296 |
| Popis: | Given the use of modified blood products (e.g. leucocyte depleted erythrocyte concentrates in SAG-mannitol, dehydrated blood powder, defibrinated blood), drawing samples from conscious animals while minimizing stress should be considered a superior option in forensics. Nevertheless, the blood portion must be well described since individual differences in quality can occur, and storage will influence blood components qualitatively and quantitatively. Cow has been discussed as a suitable source of blood supply, but current data lack hematological and full rheological perspectives. Applying our own protocol for blood withdrawal in pigs (A. Sparer et al., Forensic Science International 2020;311:110268), this project includes the respective parameters in combination with passive drip pattern experiments during refrigerated storage in multiple study arms. Cow blood displayed a constant increase in viscosity (at high shear rate: 1000 s-1), reflecting the expected reduction in red blood cell (RBC) flexibility. RBCs shrank but remained intact with very few irregular shapes, therefore there was no evidence of hemolysis. Influence of storage on stain size in passive drip pattern experiments with different substrates was minimal. However in cows, it is not hemolysis but an early change in suspension properties that indicates storage lesion. Viscosity (at low shear rate: 1 s-1) of some blood samples increased three-fold (peaking at day 14), transitioning sharply to near-Newtonian (almost shear-independent) behavior thereafter. The higher this increase in viscosity, the greater the increase in the number of satellite spatter on glass. In order to ensure high quality simulations in the future, comprehensive rheological analyses to detect gradual changes in blood pseudoplasticity should be implemented in BPA. Highlights:•Bovine RBCs shrink but remain intact during four weeks of cold storage•Influence of storage on stain size of passive drip pattern experiments is minimal •Storage lesion cannot be detected by hemolysis but by blood behavior at low flow•End point of storage is the switch towards Newtonian behavior starting at day 14•The greater the change in suspension property, the higher the rise in satellites |
| Databáze: | OpenAIRE |
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