Fungal diversity in oil palm leaves showing symptoms of Fatal Yellowing disease
Autor: | Cristine Chaves Barreto, Betania Ferraz Quirino, Ohana Y. A. Costa, Daiva Domenech Tupinambá, Jessica C. Bergmann |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Leaves Phytophthora palmivora lcsh:Medicine Plant Science Arecaceae Elaeis guineensis Trees Medicine and Health Sciences Oil Palm DNA Fungal lcsh:Science Oomycete Multidisciplinary biology Plant Anatomy Plant Fungal Pathogens Fungal Diseases Fungal genetics High-Throughput Nucleotide Sequencing Eukaryota food and beverages Biodiversity Plants 030108 mycology & parasitology Plant disease Horticulture Infectious Diseases Oomycetes Phytophthora Brazil Research Article Plant Pathogens Microbiology 03 medical and health sciences Plant Diseases lcsh:R Organisms Fungi Correction Biology and Life Sciences Plant Pathology biology.organism_classification Plant Leaves 030104 developmental biology Vegetable oil lcsh:Q Mycobiome |
Zdroj: | PLoS ONE PLoS ONE, Vol 13, Iss 1, p e0191884 (2018) |
ISSN: | 1932-6203 |
Popis: | Oil palm (Elaeis guineensis Jacq.) is an excellent source of vegetable oil for biodiesel production; however, there are still some limitations for its cultivation in Brazil such as Fatal Yellowing (FY) disease. FY has been studied for many years, but its causal agent has never been determined. In Colombia and nearby countries, it was reported that the causal agent of Fatal Yellowing (Pudrición del Cogollo) is the oomycete Phytophthora palmivora, however, several authors claim that Fatal Yellowing and Pudrición del Cogollo (PC) are different diseases. The major aims of this work were to test, using molecular biology tools, Brazilian oil palm trees for the co-occurrence of the oomycete Phytophthora and FY symptoms, and to characterize the fungal diversity in FY diseased and healthy leaves by next generation sequencing. Investigation with specific primers for the genus Phytophthora showed amplification in only one of the samples. Analysis of the fungal ITS region demonstrated that, at the genus level, different groups predominated in all symptomatic samples, while Pyrenochaetopsis and unclassified fungi predominated in all asymptomatic samples. Our results show that fungal communities were not the same between samples at the same stage of the disease or among all the symptomatic samples. This is the first study that describes the evolution of the microbial community in the course of plant disease and also the first work to use high throughput next generation sequencing to evaluate the fungal community associated with leaves of oil palm trees with and without symptoms of FY. |
Databáze: | OpenAIRE |
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