Coxiella burnetii in slaughterhouses in Brazil: A public health concern

Autor: Vanessa Cristina Pelícia, Elodie Rousset, Bruna Letícia Devidé Ribeiro, Jane Megid, Mateus de Souza Ribeiro Mioni, Karim Sidi-Boumedine, Wanderson Sirley Reis Teixeira, Richard Thiéry, Marcelo Bahia Labruna, Francisco B. Costa
Přispěvatelé: Universidade Estadual Paulista (Unesp), Univ Estadual Maranhao, Universidade de São Paulo (USP), French Agcy Food Environm & Occupat Hlth Safety
Rok vydání: 2020
Předmět:
Bacterial Diseases
Veterinary medicine
Animal Slaughter
Fluorescent Antibody Technique
Pathology and Laboratory Medicine
Geographical locations
0403 veterinary science
Medical Conditions
0302 clinical medicine
Epidemiology
Medicine and Health Sciences
Phylogeny
Animal Management
Mammals
Multidisciplinary
Geography
biology
Zoonosis
Eukaryota
Agriculture
Ruminants
04 agricultural and veterinary sciences
Bacterial Pathogens
Titer
Infectious Diseases
Veterinary Diseases
Medical Microbiology
Coxiella burnetii
Vertebrates
Medicine
Public Health
Pathogens
Q Fever
Brazil
Abattoirs
Research Article
medicine.medical_specialty
Livestock
040301 veterinary sciences
Science
030231 tropical medicine
Cattle Diseases
Q fever
Real-Time Polymerase Chain Reaction
Microbiology
03 medical and health sciences
Bovines
medicine
Animals
Microbial Pathogens
IMUNOFLUORESCÊNCIA EM ANIMAL
Public health
Organisms
Biology and Life Sciences
Outbreak
South America
bacterial infections and mycoses
medicine.disease
biology.organism_classification
Bacteremia
Amniotes
Cattle
Veterinary Science
People and places
Zoology
Zdroj: Web of Science
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
PLoS ONE, Vol 15, Iss 10, p e0241246 (2020)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0241246
Popis: Made available in DSpace on 2021-06-25T12:24:45Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-10-30 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Q fever is an important zoonosis, yet it is often neglected and can present large outbreaks, as observed in the Netherlands. In the past few years, cases of Q fever have been described in Brazil; however, the epidemiological situation of Q fever in ruminants, the main reservoir of the pathogen, is unknown in this country. Our study aimed to estimate the prevalence of C. burnetii in cattle sent to slaughterhouses using an immunofluorescence assay (IFA) and quantitative real-time PCR (qPCR). From 1515 cattle serum samples collected from nine slaughterhouses, 23.8% (360/1515) were serologically positive by IFA (cutoff titer>1:64), indicating past or recent exposure to C. burnetii infection. Among the 54 cities sampled during the study, 83.3% (45/54) had at least one seropositive animal. Subsequently, all seropositive samples were submitted to qPCR for C. burnetii DNA, and 12.2% (44/360) of the sera were qPCR positive, which indicates bacteremia and suggests active or recent infection. The results highlight the risk for abattoir workers that results from exposure to contaminated aerosols produced during slaughter procedures. Moreover, the heat maps that were construction from the positive samples demonstrate the widespread distribution of C. burnetii in the State of Sao Paulo, Brazil and denotes the need for surveillance and preventive measures to reduce the prevalence in cattle. Univ Estadual Paulista, Dept Higiene Vet & Saude Publ, Botucatu, SP, Brazil Univ Estadual Maranhao, Dept Patol, Sao Luis, Maranhao, Brazil Univ Sao Paulo, Inst Quim, Sao Paulo, SP, Brazil Univ Sao Paulo, Dept Med Vet Prevent & Saude Anim, Sao Paulo, SP, Brazil French Agcy Food Environm & Occupat Hlth Safety, Sophia Antipolis Lab, Anses, Anim Q Fever Unit, Sophia Antipolis, France Univ Estadual Paulista, Dept Higiene Vet & Saude Publ, Botucatu, SP, Brazil CAPES: 88887.137563/2017-00 CAPES: 88882.180556/2018-01 FAPESP: 2019/05300-9
Databáze: OpenAIRE
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