N,N′-Carbonyldiimidazole-mediated functionalization of superparamagnetic nanoparticles as vaccine carrier
| Autor: | Gareth M. Forde, Aswan Al-Abboodi, Cordelia Selomulya, Magdalena Plebanski, Fatin Muhammed Nawwab Al-Deen, Sue D. Xiang, Jenny Ho |
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| Rok vydání: | 2011 |
| Předmět: |
Biocompatibility
Cell Survival Ovalbumin Stereochemistry Nanoparticle Ferric Compounds Magnetics chemistry.chemical_compound Colloid and Surface Chemistry X-Ray Diffraction Chlorocebus aethiops Spectroscopy Fourier Transform Infrared Zeta potential Animals Physical and Theoretical Chemistry Magnetite Nanoparticles Trisodium citrate Magnetite Drug Carriers Vaccines Cell Death Imidazoles Temperature Surfaces and Interfaces General Medicine Hydrogen-Ion Concentration Combinatorial chemistry chemistry COS Cells Hydrodynamics Electrophoresis Polyacrylamide Gel Drug carrier Carbonyldiimidazole Biotechnology Superparamagnetism |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 83:83-90 |
| ISSN: | 0927-7765 |
| DOI: | 10.1016/j.colsurfb.2010.11.001 |
| Popis: | Particulates with specific sizes and characteristics can induce potent immune responses by promoting antigen uptake of appropriate immuno-stimulatory cell types. Magnetite (Fe(3)O(4)) nanoparticles have shown many potential bioapplications due to their biocompatibility and special characteristics. Here, superparamagnetic Fe(3)O(4) nanoparticles (SPIONs) with high magnetization value (70emug(-1)) were stabilized with trisodium citrate and successfully conjugated with a model antigen (ovalbumin, OVA) via N,N'-carbonyldiimidazole (CDI) mediated reaction, to achieve a maximum conjugation capacity at approximately 13 microgmicrom(-2). It was shown that different mechanisms governed the interactions between the OVA molecules and magnetite nanoparticles at different pH conditions. We evaluated as-synthesized SPION against commercially available magnetite nanoparticles. The cytotoxicity of these nanoparticles was investigated using mammalian cells. The reported CDI-mediated reaction can be considered as a potential approach in conjugating biomolecules onto magnetite or other biodegradable nanoparticles for vaccine delivery. |
| Databáze: | OpenAIRE |
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