Photoreceptor proteins as cancer-retina antigens
| Autor: | Dirk Schadendorf, Pavel P. Philippov, Anita Heinzelmann, Stefan B. Eichmüller, Nadine Willner, N.K. Tikhomirova, Charles De Smet, Viktor Umansky, Alexandr V. Bazhin |
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| Rok vydání: | 2007 |
| Předmět: |
Cancer Research
medicine.medical_specialty Transcription Genetic genetic structures Mice Transgenic Biology Retina Mice Antigens Neoplasm Recoverin Internal medicine medicine Arrestin Animals Humans Photoreceptor Cells Tissue Distribution RNA Messenger Eye Proteins Melanoma Autoantibodies Regulation of gene expression Photoreceptor protein eye diseases Cell biology Gene Expression Regulation Neoplastic Rhodopsin kinase Endocrinology Oncology Rhodopsin biology.protein Melanocytes sense organs Transducin Visual phototransduction |
| Zdroj: | International Journal of Cancer. 120:1268-1276 |
| ISSN: | 1097-0215 0020-7136 |
| DOI: | 10.1002/ijc.22458 |
| Popis: | Melanocytes, melanoma and photoreceptor cells are of neuroectodermal origin and have a certain sensitivity to light. In this study, we present evidence for photoreceptor proteins that are responsible for visual transduction and its regulation function as a new class of cancer antigens in melanoma. Visual rhodopsin, transducin, cGMP-phosphodiesterase 6, cGMP-dependent channels, guanylyl cyclase, rhodopsin kinase, recoverin and arrestin are expressed in melanoma and can induce antibody responses in patients. Melanocytes also express mRNA of all photoreceptor genes besides transducin, but were devoid of the corresponding protein, which was tested for rhodopsin, cGMP-phosphodiesterase, guanylyl cyclase and recoverin. Furthermore, we show for the first time that some healthy tissues express mRNA of these genes, but never protein. Expression profiles and autoantibody responses were confirmed in the MT/ret and the HGF(tg)/Ink4a(-/-) transgenic mouse melanoma models. We propose a molecular transition of cancer-retina antigens from mRNA expression in melanocytes to protein expression in melanoma. Our work provides the basis for analyzing regulation of photoreceptor gene expression in normal and malignant cells as well as possible therapeutic tumor targeting using the newly defined class of cancer-retina antigens. |
| Databáze: | OpenAIRE |
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