Phage adsorption on Enteropathogenic and Shiga Toxin-Producing Escherichia coli strains: influence of physicochemical and physiological factors
| Autor: | Andrea del Lujan Quiberoni, Cecilia Casabonne, David Damian Tomat, Claudia Balagué |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
ADSORPTION
biology RECEPTOR Chemistry medicine.disease_cause biology.organism_classification Microbiology Bacteriophage Ciencias Biológicas Biochemistry Biología Celular Microbiología medicine ESCHERICHIA COLI Escherichia coli Shiga toxin-producing Escherichia coli BACTERIOPHAGE CIENCIAS NATURALES Y EXACTAS Food Science |
| Popis: | Bacteriophages have proved to be useful tools against pathogenic Escherichia coli strains. The first step in the phage life cycle is the adsorption to the host cell surface. In the present work, three Myoviridae phages (DT1, DT5 and DT6) were used to characterize the adsorption process on three pathogenic E. coli strains, namely two Shiga-toxin producing E. coli (STEC) and one enteropathogenic E. coli (EPEC), in several conditions found on food. The influence of Na+, Mg2+, temperature, pH, periodate, proteinase K and physiological cell state on phage adsorption was investigated. The three phages evaluated showed high adsorption rates at pH 7.5 and 5.7 while they were moderate at the lowest pH evaluated (4.5). Sodium or magnesium ions were not indispensable for the adsorption of the three phages evaluated. Specifically, phage particles were adsorbed either in the presence or absence of Mg2+, while increasing Na+ concentration resulted in lower adsorption values for all the systems evaluated. Regarding temperature, phage adsorption was slightly affected at 4 °C and 50 °C, while it reached its maximum at 37 °C. Adsorption rates decreased after the thermal inactivation of cells, though, when chloramphenicol (as protein-synthesis inhibitor) was used, adsorption values on treated and untreated cells were similar. In addition, periodate was able to decrease phage adsorption, thus suggesting the receptors were carbohydrates in nature. All these results showed that the adsorption process was only partially affected and most conditions are suitable for the completion of the first step in the phage life cycle. Therefore, phages evaluated in this study can be used to prevent foodborne diseases on several food matrices since they are active in a wide range of environmental conditions. Fil: Tomat, David Damian. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Microbiologia; Argentina Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial; Argentina Fil: Casabonne, Cecilia. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Microbiologia; Argentina Fil: Balagué, Claudia. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Microbiologia; Argentina |
| Databáze: | OpenAIRE |
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