β-lactamase genes in carbapenem resistance Acinetobacter baumannii isolates from a Turkish university hospital
| Autor: | Halbay Turumtay, Cemal Sandalli, Emel Çalişkan, Umut Safiye Say Coskun, Asegul Copur Cicek |
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| Přispěvatelé: | RTEÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Çiçek, Ayşegül Çopur, Sandallı, Cemal |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Acinetobacter baumannii
Carbapenem bla(OXA-23) Turkey Resistance genes multi drug resistance Tigecycline Microbial Sensitivity Tests Microbiology Multi drug resistance beta-Lactam Resistance beta-Lactamases Hospitals University 03 medical and health sciences 0302 clinical medicine Antibiotic resistance Virology polycyclic compounds medicine Humans 030212 general & internal medicine biology resistance genes 030206 dentistry General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Multiple drug resistance Infectious Diseases Carbapenems Amikacin Colistin bacteria Parasitology Gentamicin Bla(OXA-23) Multiplex Polymerase Chain Reaction medicine.drug Acinetobacter Infections |
| Popis: | WOS: 000457877600007 PubMed: 32032023 Introduction: The spread of Acinetobacter baumannii, resistant to most of the available antimicrobial agents, is a serious health problem. The high rate of carbapenem resistance among Acinetobacter baumannii isolates is considered as a threat to public health. In this study, we aimed to determine the antibiotic resistance and related genes in carbapenem-resistant Acinetobacter baumannii isolates. Methodology: Ninety six isolates of A. baumannii were included. Antimicrobial susceptibility was performed by Phoenix Automated System and disk diffusion method. Carbapenem resistane was characterized by scrneeing of resistance genes such as bla(TEM), bla(SHV), bla(CTX-M1-2), bla(PER), bla(VEB), bla(KPC), bla(GES), bla(NDM), bla(VIM), bla(IMP) and bla(OXA23-24-51-58) using multiplex polymerase chain reaction. Results: Resistance for the levofloxacin, gentamicin, amikacin, and tigecycline were determined as 96.9%, 93.7%, 72.9% and 45.8% respectively. Colistin was the only susceptible antibiotic against all clinical isolates. All isolates were defined as multidrug resistance and of these, 31.2% were extensively drug-resistant (sensitive only to colistin). B/aoxA-51 and b/aoxA-23 genes were detected in 100% strains while bla(TEM) was found in only 2% strains. There was no amplification for the bla(SHV), bla(CTX-M1-2), bla(PER), bla(VEB), bla(KPC), bla(GES) bla(NDM), bla(VIM), bla(IMP) and bla(OXA24-58) genes. Conclusions: The high frequency of bla(OXA-23) and low frequency of bla(TEM) gene was observed that indicate prevalence of a variety of A. baumannii strains. The rates of resistance genes vary from region to region. Studies are required for the prevention and control of A. baumannii infection and to formulate the strategies of antibiotic usage. Recep Tayyip Erdogan UniversityRecep Tayyip Erdogan University [2014.102.03.02, 2014.102.03.03] The authors extend their appreciation to the Recep Tayyip Erdogan University Research Fund Grants for funding this work through the research project No: 2014.102.03.02 and 2014.102.03.03. |
| Databáze: | OpenAIRE |
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