Effects of sevoflurane general anesthesia during early pregnancy on AIM2 expression in the hippocampus and parietal cortex of Sprague-Dawley offspring rats
| Autor: | Wei Jing, Chao Lv, Shujun Shang, Yulin Zhu, Jingying Liu |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cancer Research Offspring sevoflurane general anesthesia hippocampus Morris water navigation task Hippocampus Posterior parietal cortex Hippocampal formation Sevoflurane 03 medical and health sciences symbols.namesake 0302 clinical medicine Immunology and Microbiology (miscellaneous) Medicine interferon-inducible protein AIM2 early pregnancy Glial fibrillary acidic protein biology business.industry General Medicine Articles 030104 developmental biology nervous system parietal cortex 030220 oncology & carcinogenesis Anesthesia biology.protein Nissl body symbols business medicine.drug |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-0981 |
| Popis: | The aim of the present study was to investigate the effect of exposure to sevoflurane general anesthesia during early pregnancy on interferon-inducible protein AIM2 (AIM2) expression in the hippocampus and parietal cortex of the offspring Sprague-Dawley (SD) rats. A total of 18 SD rats at a gestational age of 5-7 days were randomly divided into three groups: i) A control group (control); ii) 2-h sevoflurane general anesthesia, group 1 (S1); and iii) 4-h sevoflurane general anesthesia, group 2 (S2). The six offspring rats in each group were maintained for 30 days and assessed by Morris water maze testing. Brain specimens were collected from offspring rats 30 days after birth. Changes in the structural morphology of neurons in the hippocampus and parietal cortex were observed using hematoxylin and eosin staining. Nissl bodies in the hippocampus and parietal cortex were observed by Nissl staining. The expression of glial fibrillary acidic protein (GFAP), AIM2, CD45 and IL-1β was detected by immunohistochemistry and the protein levels of CD45, IL-1β, pro-caspase-1 and caspase-1 p10 were detected by western blotting. Compared with the control group, offspring rats in the S1 and S2 groups exhibited poor long-term learning and memory ability and experienced different degrees of damage to both the hippocampus and parietal cortex. The expression levels of GFAP, AIM2, CD45, IL-1β, caspase-1 and caspase-1 p10 in the offspring of both the S1 and the S2 groups were significantly increased (P |
| Databáze: | OpenAIRE |
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