Arabidopsis phytochelatin synthase 1, but not phytochelatin synthesis, functions in extracellular defense against multiple fungal pathogens
| Autor: | Clara Sánchez-Rodríguez, Mariola Piślewska-Bednarek, Volker Lipka, Candice Cherk, Melisa Lim, Paul Schulze-Lefert, Mónica Stein, Christine Klapprodt, Paweł Bednarek, Kian Hématy, Antonio Molina, Rene Fuchs, Erwin Grill, Shauna Somerville |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Protein moonlighting 0303 health sciences biology Myrosinase Mutant Glutathione biology.organism_classification 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Metabolic pathway chemistry Biochemistry Arabidopsis Phytochelatin Heavy metal detoxification 030304 developmental biology 010606 plant biology & botany |
| Popis: | Phytochelatin synthase (PCS) is a key component of heavy metal detoxification in plants. PCS catalyzes both the synthesis of the peptide phytochelatin from glutathione as well as the degradation of glutathione conjugates via peptidase activity. Here, we describe a hitherto uncharacterized role for PCS in disease resistance against plant pathogenic fungi. The pen4 mutant, which is allelic to cadmium insensitive 1 (cad1/pcs1) mutants, was recovered from a screen for Arabidopsis mutants with reduced resistance to the non-adapted barley fungal pathogen, Blumeria graminis f. sp. hordei. PCS1, which is found in the cytoplasm of cells of healthy plants, translocates upon pathogen attack and colocalizes with the PEN2 myrosinase on the surface of immobilized mitochondria. pcs1 and pen2 mutant plants exhibit a similar metabolic defect in the accumulation of pathogen-inducible indole glucosinolate-derived compounds, suggesting that PEN2 and PCS1 act in the same metabolic pathway. The function of PCS1 in this pathway is independent of phytochelatin synthesis and deglycination of glutathione conjugates, as catalytic-site mutants of PCS1 are still functional in indole glucosinolate metabolism. In uncovering a previously unknown function for PCS1, we reveal this enzyme to be a moonlighting protein important for plant responses to both biotic and abiotic stresses. |
| Databáze: | OpenAIRE |
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