Detection of critical antibiotic resistance genes through routine microbiome surveillance

Autor: Carl J. Schmidt, Jennifer L. Pechal, M. Eric Benbow, Heather R. Jordan, Jason W. Rosch, Zachary M. Burcham, Christopher P. Brooks
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Meticillin
Molecular biology
DNA cloning
Drug resistance
Antibiotics
Medicine and Health Sciences
education.field_of_study
Multidisciplinary
Antimicrobials
Microbiota
Human microbiome
Drugs
Drug Resistance
Microbial
Genomics
Bacterial Infections
Anti-Bacterial Agents
Medical Microbiology
Tetracyclines
Population Surveillance
Medicine
Autopsy
medicine.drug
Research Article
Science
030106 microbiology
Population
Surgical and Invasive Medical Procedures
Microbial Genomics
Biology
Microbiology
03 medical and health sciences
Antibiotic resistance
Microbial Control
medicine
Genetics
Cadaver
Humans
Microbiome
education
Sequencing Techniques
Pharmacology
Shotgun Sequencing
Bacteria
Organisms
Biology and Life Sciences
United States
Research and analysis methods
030104 developmental biology
Molecular biology techniques
Metagenomics
Genes
Bacterial
Antibiotic Resistance
Microbial genetics
Metagenome
Antimicrobial Resistance
Cloning
Zdroj: PLoS ONE
PLoS ONE, Vol 14, Iss 3, p e0213280 (2019)
ISSN: 1932-6203
Popis: Population-based public health data on antibiotic resistance gene carriage is poorly surveyed. Research of the human microbiome as an antibiotic resistance reservoir has primarily focused on gut associated microbial communities, but data have shown more widespread microbial colonization across organs than originally believed, with organs previously considered as sterile being colonized. Our study demonstrates the utility of postmortem microbiome sampling during routine autopsy as a method to survey antibiotic resistance carriage in a general population. Postmortem microbial sampling detected pathogens of public health concern including genes for multidrug efflux pumps, carbapenem, methicillin, vancomycin, and polymixin resistances. Results suggest that postmortem assessments of host-associated microbial communities are useful in acquiring community specific data while reducing selective-participant biases.
Databáze: OpenAIRE
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