Ozone responses in Arabidopsis: beyond stomatal conductance
| Autor: | Alexey Shapiguzov, Mikael Brosché, Hannes Kollist, Johanna Leppälä, Omid Safronov, Luis Orlando Morales, Dmitry Yarmolinsky, Lauri Vaahtera |
|---|---|
| Přispěvatelé: | Organismal and Evolutionary Biology Research Programme, Viikki Plant Science Centre (ViPS), Plant ROS-Signalling, Plant Biology, Molecular and Integrative Biosciences Research Programme, Plant-Fungal Interactions Group, Bioinformatics for Molecular Biology and Genomics (BMBG), Plant stress and natural variation |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Stomatal conductance THALIANA ACCESSIONS Photosystem II Transcription Genetic Physiology Arabidopsis Plastoquinone Plant Science Photosynthesis 01 natural sciences Antioxidants Electron Transport 03 medical and health sciences chemistry.chemical_compound Focus Issue on Plant Redox Biology Ozone Gene Expression Regulation Plant ABIOTIC STRESS 11. Sustainability Botany Genetics Arabidopsis thaliana TRANSCRIPTOME FLUORESCENCE Chlorophyll fluorescence GENE-EXPRESSION 030304 developmental biology 2. Zero hunger 0303 health sciences ROLES biology Cell Death Abiotic stress Botanik 11831 Plant biology biology.organism_classification OXIDATIVE BURST CELL-DEATH chemistry 13. Climate action Plant Stomata SENSITIVITY 010606 plant biology & botany |
| Zdroj: | Plant Physiol |
| Popis: | Tropospheric ozone (O3) is a major air pollutant that decreases yield of important crops worldwide. Despite long-lasting research of its negative effects on plants, there are many gaps in our knowledge on how plants respond to O3. In this study, we used natural variation in the model plant Arabidopsis (Arabidopsis thaliana) to characterize molecular and physiological mechanisms underlying O3 sensitivity. A key parameter in models for O3 damage is stomatal uptake. Here we show that the extent of O3 damage in the sensitive Arabidopsis accession Shahdara (Sha) does not correspond with O3 uptake, pointing toward stomata-independent mechanisms for the development of O3 damage. We compared tolerant (Col-0) versus sensitive accessions (Sha, Cvi-0) in assays related to photosynthesis, cell death, antioxidants, and transcriptional regulation. Acute O3 exposure increased cell death, development of lesions in the leaves, and decreased photosynthesis in sensitive accessions. In both Sha and Cvi-0, O3-induced lesions were associated with decreased maximal chlorophyll fluorescence and low quantum yield of electron transfer from Photosystem II to plastoquinone. However, O3-induced repression of photosynthesis in these two O3-sensitive accessions developed in different ways. We demonstrate that O3 sensitivity in Arabidopsis is influenced by genetic diversity given that Sha and Cvi-0 developed accession-specific transcriptional responses to O3. Our findings advance the understanding of plant responses to O3 and set a framework for future studies to characterize molecular and physiological mechanisms allowing plants to respond to high O3 levels in the atmosphere as a result of high air pollution and climate change. |
| Databáze: | OpenAIRE |
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