Upregulation of endothelial cell adhesion molecules characterizes veins close to granulomatous infiltrates in the renal cortex of cats with feline infectious peritonitis and is indirectly triggered by feline infectious peritonitis virus-infected monocytes in vitro
| Autor: | Wendy Baetens, Annelike Dedeurwaerder, Lowiese Desmarets, Hans Nauwynck, Dominique A. J. Olyslaegers, Inge D. M. Roukaerts, Gaëtan M. A. De Gryse, Sebastiaan Van Bockstael, Delphine D. Acar |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Kidney Cortex Intercellular Adhesion Molecule-1 Vascular Cell Adhesion Molecule-1 030204 cardiovascular system & hematology Biology Monocytes Feline Infectious Peritonitis Proinflammatory cytokine 03 medical and health sciences 0302 clinical medicine Virology Cell Adhesion Animals Coronavirus Feline Cell adhesion Cells Cultured Cell adhesion molecule Endothelial Cells Adhesion Leukocyte extravasation Feline infectious peritonitis Up-Regulation P-Selectin 030104 developmental biology Immunology Cats E-Selectin Cell Adhesion Molecules Selectin |
| Zdroj: | Journal of General Virology. 97:2633-2642 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/jgv.0.000585 |
| Popis: | One of the most characteristic pathological changes in cats that have succumbed to feline infectious peritonitis (FIP) is a multifocal granulomatous phlebitis. Although it is now well established that leukocyte extravasation elicits the inflammation typically associated with FIP lesions, relatively few studies have aimed at elucidating this key pathogenic event. The upregulation of adhesion molecules on the endothelium is a prerequisite for stable leukocyte-endothelial cell (EC) adhesion that necessarily precedes leukocyte diapedesis. Therefore, the present work focused on the expression of the EC adhesion molecules and possible triggers of EC activation during the development of FIP. Immunofluorescence analysis revealed that the endothelial expression of P-selectin, E-selectin, intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) was elevated in veins close to granulomatous infiltrates in the renal cortex of FIP patients compared to non-infiltrated regions and specimens from healthy cats. Next, we showed that feline venous ECs become activated when exposed to supernatant from feline infectious peritonitis virus (FIPV)-infected monocytes, as indicated by increased adhesion molecule expression. Active viral replication seemed to be required to induce the EC-stimulating activity in monocytes. Finally, adhesion assays revealed an increased adhesion of naive monocytes to ECs treated with supernatant from FIPV-infected monocytes. Taken together, our results strongly indicate that FIPV activates ECs to increase monocyte adhesion by an indirect route, in which proinflammatory factors released from virus-infected monocytes act as key intermediates. |
| Databáze: | OpenAIRE |
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