Physiological and Structural Aspects of In Vitro Somatic Embryogenesis in Abies alba Mill
| Autor: | Terézia Salaj, Bart Panis, Katarína Klubicová, Rony Swennen, Jan Salaj |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
040101 forestry
0106 biological sciences Zygote Somatic embryogenesis Forestry Embryo lcsh:QK900-989 04 agricultural and veterinary sciences Biology cryopreservation 01 natural sciences Cryopreservation cytokinins Andrology chemistry.chemical_compound chemistry Cell culture Cytokinin cytology lcsh:Plant ecology 0401 agriculture forestry and fisheries silver fir Abscisic acid 010606 plant biology & botany Explant culture |
| Zdroj: | Forests, Vol 11, Iss 1210, p 1210 (2020) Forests Volume 11 Issue 11 |
| ISSN: | 1999-4907 |
| DOI: | 10.3390/f11111210 |
| Popis: | Initiation of somatic embryogenesis from immature zygotic embryos, long-term maintenance of embryogenic tissue in vitro or by cryopreservation, as well as maturation, of somatic embryos of Abies alba Mill. are reported in this study. For the initiation of embryogenic tissues, a DCR medium containing different types of cytokinins (1 mg.L&minus 1) were tested. During three consecutive years, 61 cell lines were initiated out of 1308 explants, with initiation frequencies ranging between 0.83 and 13.33%. The type of cytokinin had no profound effect on the initiation frequency within one given year. Microscopic observations revealed presence of bipolar somatic embryos in all initiated embryogenic tissues. Besides the typical bipolar somatic embryos, huge polyembryonal complexes, as well as &ldquo twin&rdquo embryos, were observed. Maturation of somatic embryos occurred on a DCR medium supplemented by abscisic acid (10 mg.L&minus 1), polyethylene glycol (PEG-4000, 7.5%) and 3% maltose. The maturation capacity was cell-line dependent. All of the four tested cell lines produced cotyledonary somatic embryos, though at different quantities, of 16 to 252 per g of fresh weight. After germination, seedlings developed, but their further growth soon stopped after the formation of a resting bud. Altogether, seven cell lines were cryopreserved, using the slow-freezing technique. After rewarming, all tested cell lines showed regrowth rates between 81.8 and 100%. |
| Databáze: | OpenAIRE |
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