Characterization of the amnion-derived AV3 cell line for use as a model for investigation of prostaglandin-cytokine interactions in human amnion
| Autor: | S. Potter, W.R. Hansen, Murray D. Mitchell, Jeffrey A. Keelan |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Clinical Biochemistry Prostaglandin Biology Dinoprostone Cell Line chemistry.chemical_compound Fetal membrane Epidermal growth factor Internal medicine medicine Humans Amnion Epidermal Growth Factor Interleukin-6 Tumor Necrosis Factor-alpha Interleukin-8 Interleukin Membrane Proteins Cell Biology Isoenzymes Endocrinology medicine.anatomical_structure Cytokine chemistry Eicosanoid Cyclooxygenase 2 Prostaglandin-Endoperoxide Synthases embryonic structures Phorbol Prostaglandins Cytokines Tetradecanoylphorbol Acetate Inflammation Mediators Interleukin-1 |
| Zdroj: | ResearcherID |
| ISSN: | 0952-3278 |
| Popis: | Increased production of prostaglandins and cytokines by amnion, particularly prostaglandin (PG) E-2, interleukin (IL)-6 and IL-8, is thought to be an important event in infection-associated preterm labour. We characterized the amnion-derived AV3 cell line to determine its appropriateness as a model for investigation of the regulation of amnion cytokine and PG production. Amnion-derived AV3 cells were treated with tumour necrosis factor-alpha (TNF-alpha, interleukin-1 beta (IL-1 beta), epidermal growth factor (EGF) and phorbol 12-myristate 13-acetate (PMA) and IL-6, IL-8 and prostaglandin production was determined by immunoassay. Production of IL-6 and IL-8 rose dramatically with all treatments. PGE(2), but not PGF(2 alpha) or 6-keto- PGF(1 alpha), biosynthesis was also increased in a concentration- dependent manner with all treatments. A rapid increase in PGHS-2 (but not PGHS-1) mRNA expression was observed in response to TNF-alpha and IL-1 beta. We conclude that the AV3 cell line inflammatory response profile is similar to those observed in primary amnion and other amnion-derived cell lines, and is an appropriate model for human amnion. (C) 1999 Harcourt Publishers Ltd. |
| Databáze: | OpenAIRE |
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