In vivo Synthesis of Cyclic-di-GMP Using a Recombinant Adenovirus Preferentially Improves Adaptive Immune Responses Against Extracellular Antigens¶
| Autor: | Christopher M. Waters, Benjamin J. Koestler, Eric L. Bruger, Andrea Amalfitano, Cristiane Pereira-Hicks, Fadel S. Alyaqoub, Sarah Godbehere, Yasser A. Aldhamen, Sergey S. Seregin |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Enzyme-Linked Immunospot Assay T cell medicine.medical_treatment Immunology Blotting Western Genetic Vectors Enzyme-Linked Immunosorbent Assay Biology Adaptive Immunity Article Adenoviridae 03 medical and health sciences Mice 0302 clinical medicine Immune system Antigen Adjuvants Immunologic Transduction Genetic medicine Immunology and Allergy Animals Antigens Cyclic GMP Mice Inbred BALB C Innate immune system Immunotherapy Acquired immune system Flow Cytometry Cell biology Adenovirus vaccine Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Adjuvant 030215 immunology medicine.drug |
| Popis: | There is a compelling need for more effective vaccine adjuvants to augment induction of Ag-specific adaptive immune responses. Recent reports suggested the bacterial second messenger bis-(3′–5′)-cyclic-dimeric-guanosine monophosphate (c-di-GMP) acts as an innate immune system modulator. We recently incorporated a Vibrio cholerae diguanylate cyclase into an adenovirus vaccine, fostering production of c-di-GMP as well as proinflammatory responses in mice. In this study, we recombined a more potent diguanylate cyclase gene, VCA0848, into a nonreplicating adenovirus serotype 5 (AdVCA0848) that produces elevated amounts of c-di-GMP when expressed in mammalian cells in vivo. This novel platform further improved induction of type I IFN-β and activation of innate and adaptive immune cells early after administration into mice as compared with control vectors. Coadministration of the extracellular protein OVA and the AdVCA0848 adjuvant significantly improved OVA-specific T cell responses as detected by IFN-γ and IL-2 ELISPOT, while also improving OVA-specific humoral B cell adaptive responses. In addition, we found that coadministration of AdVCA0848 with another adenovirus serotype 5 vector expressing the HIV-1–derived Gag Ag or the Clostridium difficile–derived toxin B resulted in significant inhibitory effects on the induction of Gag and toxin B–specific adaptive immune responses. As a proof of principle, these data confirm that in vivo synthesis of c-di-GMP stimulates strong innate immune responses that correlate with enhanced adaptive immune responses to concomitantly administered extracellular Ag, which can be used as an adjuvant to heighten effective immune responses for protein-based vaccine platforms against microbial infections and cancers. |
| Databáze: | OpenAIRE |
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