Dissecting sex pheromone communication of Mythimna separata (Walker) in North China from receptor molecules and antennal lobes to behavior
| Autor: | Nan-Ji Jiang, Chen-Zhu Wang, Ling-Qiao Huang, Meng Xu, Han Wu, Rui Tang, Chao Ning |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Arthropod Antennae Male China Patch-Clamp Techniques Xenopus Population Biology Moths 01 natural sciences Biochemistry Transcriptome 03 medical and health sciences Mythimna separata Sexual Behavior Animal medicine Animals Sex Attractants education Receptor Molecular Biology 030304 developmental biology 0303 health sciences education.field_of_study biology.organism_classification Cell biology Electrophysiological Phenomena 010602 entomology medicine.anatomical_structure Insect Science Sex pheromone Oocytes Pheromone Antennal lobe Female |
| Zdroj: | Insect biochemistry and molecular biology. 111 |
| ISSN: | 1879-0240 |
| Popis: | The Oriental armyworm, Mythimna separata, has been described to emit geographic population-specific sex pheromones, with either Z11-16:Ald or Z11-16:Ac as the major component. Using a comprehensive set of electrophysiological, behavioral, and genetic analyses, we study the sex pheromone communication of M. separata in North China from pheromone receptors and antennal lobe to behavior. GC-EAD results show that Z11-16:Ald is the only compound eliciting electrophysiological responses in pheromone gland extracts. Further in vivo optical imaging studies reveal that Z11-16:Ald activates the cumulus of the MGC and show dose-dependent responses. The wind tunnel tests demonstrate that Z11-16:Ald alone is sufficient to induce the entire sequence of male sexual behaviors. Transcriptome and q-PCR results show that MsepOR3 is specifically and abundantly expressed in male antennae. By using the Xenopus oocytes and two-electrode voltage-clamp recording, we finally validate that the oocytes expressing MsepOR3/ORco gave dose dependent responses to Z11-16:Ald. We suggest single Z11-16:Ald could be used for monitoring the population of M. separata in North China. |
| Databáze: | OpenAIRE |
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