Pre-implantation genetic screening using fluorescence in situ hybridization in couples of Indian ethnicity: Is there a scope?
| Autor: | Kundanbala Desai, Shailaja Gada Saxena, Lata Shewale, Prabhat Ranjan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Gynecology
Pregnancy medicine.medical_specialty pre-implantation genetic diagnosis In vitro fertilisation genetic counseling medicine.diagnostic_test Genetic counseling medicine.medical_treatment Context (language use) Biology medicine.disease lcsh:Gynecology and obstetrics Reproductive Medicine medicine pre-implantation genetic screening Original Article Advanced maternal age fluorescence in situ hybridization Twin Pregnancy Aneuploidy testing lcsh:RG1-991 Fluorescence in situ hybridization Unexplained infertility |
| Zdroj: | Journal of Human Reproductive Sciences, Vol 7, Iss 1, Pp 25-29 (2014) Journal of Human Reproductive Sciences |
| ISSN: | 1998-4766 0974-1208 |
| Popis: | Context: There is a high incidence of numerical chromosomal aberration in couples with repeated in vitro fertilization (IVF) failure, advanced maternal age, repeated unexplained abortions, severe male factor infertility and unexplained infertility. Pre-implantation genetic screening (PGS), a variant of pre-implantation genetic diagnosis, screens numerical chromosomal aberrations in couples with normal karyotype, experiencing poor reproductive outcome. The present study includes the results of the initial pilot study on 9 couples who underwent 10 PGS cycles. Aim: The aim of the present study was to evaluate the beneficial effects of PGS in couples with poor reproductive outcome. Settings and Design: Data of initial 9 couples who underwent 10 PGS for various indications was evaluated. Subjects and Methods: Blastomere biopsy was performed on cleavage stage embryos and subjected to two round fluorescence in situ hybridization (FISH) testing for chromosomes 13, 18, 21, X and Y as a two-step procedure. Results: Six of the 9 couples (10 PGS cycles) conceived, including a twin pregnancy in a couple with male factor infertility, singleton pregnancies in a couple with secondary infertility, in three couples with adverse obstetric outcome in earlier pregnancies and in one couple with repeated IVF failure. Conclusion: In the absence of availability of array-comparative genomic hybridization in diagnostic clinical scenario for PGS and promising results with FISH based PGS as evident from the current pilot study, it is imperative to offer the best available services in the present scenario for better pregnancy outcome for patients. |
| Databáze: | OpenAIRE |
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