Differential behaviour of epicardial adipose tissue-secretomes with high and low orosomucoid levels from patients with cardiovascular disease in H9C2 cells
| Autor: | Marinela Couselo, Rubén Fandiño-Vaquero, Ángel Fernández-Trasancos, María Cebro, José Ramón González-Juanatey, Juan Sierra, Sonia Eiras, Susana B. Bravo, Isabel Moscoso, Ricardo Lage |
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| Rok vydání: | 2015 |
| Předmět: |
Male
medicine.medical_specialty Cell Orosomucoid Apoptosis Bioinformatics Biochemistry Flow cytometry Cell Line Endocrinology Western blot Annexin Internal medicine medicine Animals Humans Annexin A5 Molecular Biology Aged Aged 80 and over medicine.diagnostic_test biology Chemistry Caspase 3 Acute-phase protein Middle Aged Flow Cytometry Cell Hypoxia Rats medicine.anatomical_structure Secretory protein Adipose Tissue Animals Newborn Cardiovascular Diseases biology.protein Female Pericardium Myoblasts Cardiac |
| Zdroj: | Molecular and cellular endocrinology. 416 |
| ISSN: | 1872-8057 |
| Popis: | Epicardial adipose tissue releases orosomucoid (ORM), an acute phase protein with multiple modulatory and protective properties. We aimed to identify the effect of EAT-supernatants according to their ORM levels on H9C2 cells. H9C2 were cultured with EAT-secretomes or ORM protein itself on a Real-Time Cell Analyser. Secretome proteins identification was performed by LC-mass spectrometry according to their ORM levels. Two of them were validated by ELISA in EAT-supernatants from 42 patients. ORM effect on H9C2 and neonatal rat cardiomyocytes apoptosis under hypoxia with or without fatty acid treatment was determined by Annexin-V flow cytometry measurement. Caspase-3 expression levels were determined by western blot in H9C2. Our results showed a differential effect of EAT-secretomes according their ORM levels. Although additional secreted proteins can contribute to their beneficial effects, ORM reduced hypoxia-induced apoptosis through caspase-3 inhibition. Our data showed the cardioprotective role of ORM and suggest that its quantification on EAT secretomes might help us to find new secreted factors with a cardioprotective role. |
| Databáze: | OpenAIRE |
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