Neuroprotective effect of ischemic preconditioning via modulating the expression of cerebral miRNAs against transient cerebral ischemia in diabetic rats

Autor: Ozgen Altintas M, Talip Asil, Meltem Kumaş, Ozge Altintas
Přispěvatelé: KUMAŞ, Meltem
Rok vydání: 2016
Předmět:
Blood Glucose
Brain Infarction
Male
0301 basic medicine
Time Factors
Ischemia
ALTINTAS O.
Altintas M. O.
Kumas M.
Asil T.
-Neuroprotective effect of ischemic preconditioning via modulating the expression of cerebral miRNAs against transient cerebral ischemia in diabetic rats-
NEUROLOGICAL RESEARCH
cilt.38
ss.1003-1011
2016
Pharmacology
Neuroprotection
Functional Laterality
Statistics
Nonparametric
Streptozocin
Brain Ischemia
Diabetes Mellitus
Experimental
Rats
Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Diabetes mellitus
medicine
Animals
cardiovascular diseases
Ischemic Preconditioning
Stroke
business.industry
Body Weight
Glutamate receptor
General Medicine
medicine.disease
Rats
Disease Models
Animal
MicroRNAs
030104 developmental biology
Aquaporin 4
Gene Expression Regulation
Neurology
Anesthesia
Toxicity
Ischemic preconditioning
Neurology (clinical)
Nervous System Diseases
business
030217 neurology & neurosurgery
Zdroj: Neurological Research. 38:1003-1011
ISSN: 1743-1328
0161-6412
DOI: 10.1080/01616412.2016.1232013
Popis: In this study, we aimed to evaluate the effect of the Ischemic preconditioning (IPreC) on the expression profile of cerebral miRNAs against stroke by induced transient middle cerebral artery occlusion (MCAo) in diabetic rats.Eighty male Spraque Dawley rats were allocated to eight groups. In order to evaluate the expression profile of miRNAs, we induced transient MCAo seven days after STZ-induced diabetes (DM). Also we performed IPreC 72 h before transient MCAo to assess whether IPreC could have a neuroprotective effect against ischemia-reperfusion injury.The general characteristics of STZ-treated rats included reduced body weight and elevated blood glucose levels compared to non-diabetic ones. We demonstrated that miRNA expression profiles, which are determined for biological functions such as aquaporin 4 formation (miR-29b-2, miR-124a-3p, miR-130a, miR-223 and miR-320a), glutamate toxicity (miR107, miR-145, miR-223), salvageable ischemic area (miR-9a, miR-19b, miR-29b-2, miR-341, miR-339-5p, miR-15-5p, miR-99b-5p), and neoangiogenesis (let-7f-5p, miR-126a and miR-322-3p), were regulated following IPreC. Ischemic preconditioning before cerebral ischemia significantly reduced infarction size compared with the other groups [IPreC + MCAo (27 ± 11 mmThe study results revealed the neuroprotective effects of ischemic preconditioning, supported with the upregulated pro-survival miRNAs in MCA infarcts.
Databáze: OpenAIRE
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