Aurones and Flavonols from Coreopsis lanceolata L. Flowers and Their Anti-Oxidant, Pro-Inflammatory Inhibition Effects, and Recovery Effects on Alloxan-Induced Pancreatic Islets in Zebrafish
| Autor: | Nam-In Baek, Tong Ho Kang, Dae-Ok Kim, Dae Young Lee, Seon Min Oh, Trong Nguyen Nguyen, Youn Hee Nam, Hyoung-Geun Kim, Min-Ho Lee, Young Sung Jung |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Coreopsis lanceolata
Flavonoid RAW 264.7 Anti-Inflammatory Agents Pharmaceutical Science Organic chemistry Flowers antioxidant capacity High-performance liquid chromatography Article Antioxidants Analytical Chemistry Cell Line chemistry.chemical_compound Islets of Langerhans Mice Flavonols QD241-441 Alloxan Drug Discovery Animals Humans heterocyclic compounds flavonoid Physical and Theoretical Chemistry Benzofurans chemistry.chemical_classification Flavonoids Chromatography pancreatic islets Plant Extracts fungi food and beverages Biological activity zebrafish anti-inflammation carbohydrates (lipids) Leptosidin RAW 264.7 Cells chemistry Chemistry (miscellaneous) Isoquercetin Molecular Medicine Astragalin Coreopsis Reactive Oxygen Species |
| Zdroj: | Molecules, Vol 26, Iss 6098, p 6098 (2021) Molecules Volume 26 Issue 20 |
| ISSN: | 1420-3049 |
| Popis: | (1) Background: Many flavonoids have been reported to exhibit pharmacological activity a preparatory study confirmed that Coreopsis lanceolata flowers (CLFs) contained high flavonoid structure content (2) Methods: CLFs were extracted in aqueous methanol (MeOH:H2O = 4:1) and fractionated into acetic ester (EtOAc), normal butanol (n-BuOH), and H2O fractions. Repeated column chromatographies for two fractions led to the isolation of two aurones and two flavonols (3) Results: Four flavonoids were identified based on a variety of spectroscopic data analyses to be leptosidin (1), leptosin (2), isoquercetin (3), and astragalin (4), respectively. This is the first report for isolation of 2–4 from CLFs. High-performance liquid chromatography (HPLC) analysis determined the content levels of compounds 1–4 in the MeOH extract to be 2.8 ± 0.3 mg/g (1), 17.9 ± 0.9 mg/g (2), 3.0 ± 0.2 mg/g (3), and 10.9 ± 0.9 mg/g (4), respectively. All isolated compounds showed radical scavenging activities and recovery activities in Caco-2, RAW264.7, PC-12, and HepG2 cells against reactive oxygen species. MeOH extract, EtOAc fraction, and 1–3 suppressed NO formation in LPS-stimulated RAW 264.7 cells and decreased iNOS and COX-2 expression. Furthermore, all compounds recovered the pancreatic islets damaged by alloxan treatment in zebrafish (4) Conclusions: The outcome proposes 1–4 to serve as components of CLFs in standardizing anti-oxidant, pro-inflammatory inhibition, and potential anti-diabetic agents. |
| Databáze: | OpenAIRE |
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