Gut Microbes Egested During Bites of Infected Sand flies Augment Severity of Leishmaniasis Via Inflammasome-Derived IL-1β
| Autor: | Tiago D. Serafim, Claudio Meneses, Lais Pereira, Robert Duncan, Amritanshu B. Joshi, Waldionê de Castro, Hamide Aslan, Parna Bhattacharya, Shaden Kamhawi, Subir Karmakar, Shannon Townsend, Anderson B. Guimarães-Costa, Alec Perkins, Iliano V. Coutinho-Abreu, Fabiano Oliveira, Hira L. Nakhasi, Morgan Karetnick, Nevien Ismail, Jesus G. Valenzuela, Ranadhir Dey |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Inflammasomes Neutrophils Interleukin-1beta Leishmania donovani Biology Gut flora Microbiology Article Mice 03 medical and health sciences 0302 clinical medicine Cricetinae Virology NLR Family Pyrin Domain-Containing 3 Protein parasitic diseases medicine Parasite hosting Animals Humans Leishmania Mice Inbred BALB C Antiparasitic Agents Insect Bites and Stings Inflammasome Leishmaniasis Midgut medicine.disease biology.organism_classification Gastrointestinal Microbiome Insect Vectors 030104 developmental biology Visceral leishmaniasis Neutrophil Infiltration Leishmaniasis Visceral Female Parasitology Psychodidae 030217 neurology & neurosurgery medicine.drug |
| Popis: | Leishmania donovani parasites are the cause of visceral leishmaniasis and are transmitted by bites from phlebotomine sand flies. A prominent feature of vector-transmitted Leishmania is the persistence of neutrophils at bite sites, where they protect captured parasites, leading to enhanced disease. Here, we demonstrate that gut microbes from the sand fly are egested into host skin alongside Leishmania parasites. The egested microbes trigger the inflammasome, leading to a rapid production of interleukin-1β (IL-1β), which sustains neutrophil infiltration. Reducing midgut microbiota by pretreatment of Leishmania-infected sand flies with antibiotics or neutralizing the effect of IL-1β in bitten mice abrogates neutrophil recruitment. These early events are associated with impairment of parasite visceralization, indicating that both gut microbiota and IL-1β are important for the establishment of Leishmania infections. Considering that arthropods harbor a rich microbiota, its potential egestion after bites may be a shared mechanism that contributes to severity of vector-borne disease. |
| Databáze: | OpenAIRE |
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