Hemoregulatory peptide (HP5b) dimer effects on normal and malignant cells in culture
| Autor: | T. Kalland, S. Frostad, Ole Didrik Laerum, A. Aakvaag |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
medicine.medical_specialty
Cell type Stromal cell Lymphoma Polymers Molecular Sequence Data Bone Marrow Cells Breast Neoplasms Biology Lymphocyte Activation Colony-Forming Units Assay Mice Bone Marrow Internal medicine Neoplasms medicine Tumor Cells Cultured Animals Amino Acid Sequence Cells Cultured Colony-forming unit Mice Inbred C3H Cell growth Brain Neoplasms Carcinoma Ductal Breast Cell Biology 3T3 Cells Glioma Hematopoietic Stem Cells Molecular biology Lymphocyte Subsets Pyrrolidonecarboxylic Acid Mice Inbred C57BL Haematopoiesis Granulocyte macrophage colony-stimulating factor Endocrinology Cell culture Molecular Medicine Female Interleukin-3 Myelopoiesis Glioblastoma Oligopeptides Developmental Biology medicine.drug |
| Zdroj: | Stem cells (Dayton, Ohio). 11(4) |
| ISSN: | 1066-5099 |
| Popis: | The dimer of the hemoregulatory peptide HP5b has been investigated for biological effects on various cell types in culture including mouse granulocyte-macrophage colony forming units (CFU-GM) from agar and murine long-term bone marrow culture (LTBMC). While CFU-GM were significantly stimulated in both systems, mitogen activation of mouse T, B and natural killer (NK) cells was not affected. Peptide treated mouse 3T3 fibroblasts reached a higher saturation density than controls; otherwise no effect was seen. A series of malignant cell lines was also tested. On a human glioblastoma cell line (GaMg) and rat glioma cell line (BT5C) a slight but significant stimulatory effect was found, while human mammary carcinoma cells (MCF7) were not affected. On SC1 mouse lymphoma cells a slight stimulation of cell growth was seen during the first part of exponential growth. Since HP5b acts as a stimulator for stromal cell secretion of other growth factors, supernatants from a human bone marrow stromal cell line stimulated with HP5b were tested on various cell lines. The effects of the supernatants on cell growth of the tested cell lines were not affected by HP5b treatment. Taken together with available in vivo data, the results indicate that the hemoregulatory peptide is a selective stimulator of myelopoiesis. |
| Databáze: | OpenAIRE |
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