Molecular Surveillance of EHV-1 Strains Circulating in France during and after the Major 2009 Outbreak in Normandy Involving Respiratory Infection, Neurological Disorder, and Abortion

Autor: Christel Marcillaud-Pitel, Loïc Legrand, Romain Paillot, Marie Garvey, Marc Foursin, Peggy Moreau, Gabrielle Sutton, Virginie Maisonnier, Christine Fortier, Ann Cullinane, Annick Gryspeerdt, Stéphane Pronost, Marion Jourdan
Přispěvatelé: LABÉO, Pôle d’analyses et de recherche de Normandie (LABÉO), Biologie, génétique et thérapies ostéoarticulaires et respiratoires (BIOTARGEN), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Irish Equine Centre (IEC), Réseau d'Epidémio-Surveillance en Pathologie Équine (RESPE), Clinique Équine de la Boisrie, Equi Focus Point Belgium
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Male
Equine herpesvirus 1
lcsh:QR1-502
Disease
Abortion
phylogeny
mlst
neuropathogenic strain
lcsh:Microbiology
Disease Outbreaks
0403 veterinary science
Belgium
Respiratory Tract Infections
biology
Phylogenetic tree
Respiratory infection
orf30
04 agricultural and veterinary sciences
Herpesviridae Infections
Abortion
Veterinary
respiratory disease
3. Good health
Infectious Diseases
equine herpesvirus type 1
Female
France
Herpesvirus 1
Equid
040301 veterinary sciences
myeloencephalopathy
Virus
Article
03 medical and health sciences
Open Reading Frames
Virology
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
Animals
Horses
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
outbreak
Outbreak
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
biology.organism_classification
abortion
United Kingdom
030104 developmental biology
DNA
Viral
Multilocus sequence typing
Horse Diseases
Nervous System Diseases
Multilocus Sequence Typing
Zdroj: Viruses, Vol 11, Iss 10, p 916 (2019)
Viruses
Viruses, MDPI, 2019, 11 (10), pp.916. ⟨10.3390/v11100916⟩
Volume 11
Issue 10
ISSN: 1999-4915
DOI: 10.3390/v11100916⟩
Popis: International audience; Equine herpesvirus 1 (EHV-1) is an Alphaherpesvirus infecting not only horses but also other equid and non-equid mammals. It can cause respiratory distress, stillbirth and neonatal death, abortion, and neurological disease. The different forms of disease induced by EHV-1 infection can have dramatic consequences on the equine industry, and thus the virus represents a great challenge for the equine and scientific community. This report describes the progress of a major EHV-1 outbreak that took place in Normandy in 2009, during which the three forms of disease were observed. A collection of EHV-1 strains isolated in France and Belgium from 2012 to 2018 were subsequently genetically analysed in order to characterise EHV-1 strain circulation. The open reading frame 30 (ORF30) non-neuropathogenic associated mutation A2254 was the most represented among 148 samples analysed in this study. ORF30 was also sequenced for 14 strains and compared to previously published sequences. Finally, a more global phylogenetic approach was performed based on a recently described Multilocus Sequence Typing (MLST) method. French and Belgian strains were clustered with known strains isolated in United Kingdom and Ireland, with no correlation between the phylogeny and the time of collection or location. This new MLST approach could be a tool to help understand epidemics in stud farms.
Databáze: OpenAIRE
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