Characterizing aging, mild cognitive impairment, and dementia with blood-based biomarkers and neuropsychology
| Autor: | Martin Kleinschmidt, Jürgen Erich Metzner, Claudia Göttlich, Hans-Ulrich Demuth, Bernd Leplow, Daniel Bittner, Robby Schoenfeld |
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| Přispěvatelé: | Publica |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
Apolipoprotein E Gerontology Oncology autoantibodies amyloid-v neuropsychology Neuropsychological Tests Activities of Daily Living Neuropsychological assessment Young adult Aged 80 and over medicine.diagnostic_test General Neuroscience Neuropsychology Cognition General Medicine Middle Aged Alzheimer's disease Psychiatry and Mental health Clinical Psychology Cytokines Female Psychology ApoE Adult medicine.medical_specialty Adolescent Young Adult Apolipoproteins E mild cognitive impairment blood Internal medicine medicine Humans Dementia Cognitive Dysfunction Aged Analysis of Variance Memory Disorders Amyloid beta-Peptides aging Autoantibody Case-control study medicine.disease Cross-Sectional Studies Case-Control Studies Geriatrics and Gerontology Biomarkers |
| Popis: | Background: Current treatment in Alzheimer’s disease (AD) is initiated at a stage where the brain already has irreversible structural deteriorations. Therefore, the concept of treatment prior to obvious cognitive deficits has become widely accepted, and simple biochemical tests to discriminate normal aging from prodromal or demented stages are now common practice. Objective: The objective of the study was the differentiation of controls, mild cognitive impairment (MCI) and AD patients by novel blood-based assays in combination with neuropsychological tests. Methods: In a cross-sectional study, 143 subjects aged 18 to 85 years were recruited. All participants were classified by a comprehensive neuropsychological assessment. Blood samples were analyzed for several amyloid-β (Aβ) species, pro-inflammatory markers, anti-Aβ autoantibodies, and ApoE allele status, respectively. Results: Plasma Aβ1-42 was significantly decreased in MCI and AD compared to age-matched controls, whereas Aβ1-40 did not differ, but increases with age in healthy controls. The Aβ1-42 to Aβ1-40 ratio was stepwise decreased from age-matched controls via MCI to AD, and shows a clear correlation with memory scores. Reduced Aβ1-42 and Aβ1-42 to Aβ1-40 ratio have strongly correlated with carrying ApoE ɛ4 allele. Autoantibodies against pyroglutamate-modified Aβ, but only a certain subclass, were significantly decreased in AD compared to MCI and age-matched controls, whereas autoantibodies against the unmodified N-terminus of Aβ did not differ. Conclusion: Comprehensive sample preparation and assay standardization enable reliable usage of plasma Aβ for diagnosis of MCI and AD. Anti-pGlu-Aβ autoantibodies correlate with cognition, but not with ApoE, supporting the associated plasma Aβ analysis with additional and independent information. |
| Databáze: | OpenAIRE |
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