Detection of Stress Hormone in the Milk for Animal Welfare Using QCM Method
| Autor: | Kiyoshi Akiyama, Katsuji Uetake, Takeshi Ito, Akihisa Tsuchiya, Koji Suzuki, Nobuyoshi Aoki, Satoru Kaneko |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Chromatography Article Subject Chemistry 0402 animal and dairy science Analytical chemistry 04 agricultural and veterinary sciences Quartz crystal microbalance 040201 dairy & animal science Stress hormone Frequency difference Nonspecific adsorption Stress marker Stress (mechanics) 03 medical and health sciences 030104 developmental biology Control and Systems Engineering lcsh:Technology (General) lcsh:T1-995 Electrical and Electronic Engineering Instrumentation Cortisol level |
| Zdroj: | Journal of Sensors, Vol 2017 (2017) |
| ISSN: | 1687-7268 1687-725X |
| DOI: | 10.1155/2017/6486891 |
| Popis: | We developed a rapid and sequential analysis system to determine stress marker in the milk. One of the famous stress markers, cortisol, was detected using our method. Quartz crystal microbalance (QCM) method with a twin sensor was used in this study. One channel detected stress marker corresponding to antigen-antibody interaction and the other channel was used as a reference to remove environmental influences. Although nonspecific adsorption was monitored on each channel, frequency difference between them was within a few Hz on the injection of sample solution. One determination cycle including regeneration step could be performed within 10 minutes. The system could detect the cortisol level from 0.1 pg/mL to 100 pg/mL. These results show that our system has a potential to check the daily feeding condition for cows in terms of animal welfare. |
| Databáze: | OpenAIRE |
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