| Autor: |
Khan, Muhammad Babar, Ruggieri, Rosamaria, Eesha Jamil, Tran, Nhan L., Gonzalez, Camila, Mugridge, Nancy, Gao, Steven, MacDiarmid, Jennifer, Himanshu Brahmbhatt, Sarkaria, Jann N., Boockvar, John, Symons, Marc |
| Rok vydání: |
2021 |
| DOI: |
10.6084/m9.figshare.14313794 |
| Popis: |
Additional file 6: Figure S3. miR-34a downregulates multiple therapeutic resistance genes. A. Transfection with miR-34a significantly increases miR-34a expression in glioblastoma primary cultures. GBM6, GBM118 and GBM126 cells were transfected with 30 nM control miRNA or miR-34a. Total RNA was isolated 48 h post transfection. RNU-6 was used as housekeeping gene and relative expression calculations were made according to the Livak method. Data show mean ± SD of three technical replicates. B. miR-34a reduces to expression of multiple therapeutic resistance genes in glioblastoma. RTqPCR experiments were performed with independent primers to verify the results of the PCR array. In some instances, expression of genes could not be detected in one or more cultures by the PCR. The corresponding bars are left missing from the figure. RNU-6 was used as housekeeping gene and relative expression calculations were made according to the Livak method. Data was normalized to non-transfected controls and show mean ± SD of three technical replicates. *p 0.05 |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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