Performance of the CRAFTi portable fluorometer comparing with the HPLC method for determining serum retinol
| Autor: | Neal E. Craft, Emorn Wasantwisut, Laksana Chaimongkol, John E. Estes, Pattanee Winichagoon, Siwaporn Pinkaew, Harold C. Furr |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Hplc analysis Chromatography Vitamin A Deficiency Chemistry Clinical Biochemistry Serum retinol Retinol Nutritional status General Medicine Serum samples Sensitivity and Specificity High-performance liquid chromatography chemistry.chemical_compound Fluorometer Humans Female Fluorometry Child Vitamin A Hplc method Chromatography High Pressure Liquid |
| Zdroj: | Clinical Biochemistry. 44:1030-1032 |
| ISSN: | 0009-9120 |
| DOI: | 10.1016/j.clinbiochem.2011.05.023 |
| Popis: | Objective To determine the performance of a portable fluorometer for measuring serum retinol (SR) concentration. Design and methods Serum samples were obtained from 75 factory worker women and 143 school children. SR concentration was quantified using a portable fluorometer (‘CRAFTi’) and HPLC analysis. Results SR by HPLC (1.23 ± 0.43 μmol/L) and CRAFTi (1.16 ± 0.46 μmol/L) was significantly correlated. Sensitivity and specificity were 85.3% and 78.0% (cutoff of 1.05 μmol/L). Kappa statistics showed moderate agreement. Conclusions CRAFTi portable fluorometer is a promising field-friendly tool for screening vitamin A deficiency. |
| Databáze: | OpenAIRE |
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