Tigecycline has no effect on cytokine release in an ex vivo endotoxin model of human whole blood
| Autor: | Christiane Thallinger, Friederike Traunmüller, Stanislava Tzaneva, Johann Hausdorfer, Christopher Lambers, Thomas Kampitsch, Christian Joukhadar, Georg Endler |
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| Rok vydání: | 2008 |
| Předmět: |
Microbiology (medical)
Lipopolysaccharides medicine.medical_treatment Minocycline Tigecycline Biology Pharmacology In Vitro Techniques Proinflammatory cytokine In vivo medicine Humans Pharmacology (medical) Cells Cultured Antibacterial agent Whole blood Interleukin General Medicine Infectious Diseases Cytokine Blood Immunology Cytokines Ex vivo medicine.drug |
| Zdroj: | International journal of antimicrobial agents. 33(6) |
| ISSN: | 1872-7913 |
| Popis: | In previous studies, tetracyclines have been shown to decrease the release of cytokines in experimental settings of endotoxaemia. Tigecycline is the first member of the closely related glycylglycines and, due to its broad antimicrobial spectrum, it is considered useful in the treatment of sepsis. We therefore tested its ability to influence the concentrations of the proinflammatory cytokines interleukin (IL)-1beta, tumour necrosis factor-alpha (TNFalpha) and IL-6 in an established ex vivo model of human endotoxaemia. Whole blood from ten healthy volunteers was incubated with either saline (negative control), tigecycline (1 microg/mL [therapeutic concentration] or 100 microg/mL [supratherapeutic concentration]), lipopolysaccharide (LPS; 50 pg/mL, control) or a combination of tigecycline plus LPS (test group). Concentrations of IL-1beta, TNFalpha and IL-6 in the supernatant were measured using commercially available enzyme-linked immunosorbent assay (ELISA) kits. As expected, incubation with LPS significantly increased the cytokine concentrations in whole blood compared with baseline (P |
| Databáze: | OpenAIRE |
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