Paracetamol inhibits nitric oxide synthesis in murine spinal cord slices
| Autor: | Ian Kitchen, Geoffrey D. Clarke, Susanna M.O. Hourani, Ian Bailey, Nick J. Toms, Lisa Godfrey |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Male
Cerebellum Arginine Analgesic Central nervous system Glutamic Acid In Vitro Techniques Pharmacology Nitric Oxide Nitric oxide Mice chemistry.chemical_compound Caffeine health services administration medicine Citrulline Animals Enzyme Inhibitors Acetaminophen Dose-Response Relationship Drug Chemistry organic chemicals digestive oral and skin physiology Analgesics Non-Narcotic Spinal cord NG-Nitroarginine Methyl Ester medicine.anatomical_structure Spinal Cord Biochemistry Central Nervous System Stimulants Nitric Oxide Synthase |
| Zdroj: | European Journal of Pharmacology. 562:68-71 |
| ISSN: | 0014-2999 |
| DOI: | 10.1016/j.ejphar.2007.01.075 |
| Popis: | Paracetamol is an effective analgesic but its mechanism of action is unclear. We investigated the effect of paracetamol and the analgesic adjuvant caffeine on the activity of NO synthase in mouse spinal cord and cerebellar slices in vitro, by measuring the conversion of [(3)H]arginine to [(3)H]citrulline. Paracetamol (100 microM) had no effect on NO synthase activity in cerebellum, but in the spinal cord both paracetamol (100 microM) and caffeine (30 microM) attenuated glutamate (5 mM)-induced [(3)H]citrulline production and in combination they abolished it. In conclusion paracetamol inhibits spinal cord NO synthesis and this may be related to its analgesic effects. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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