GEBF-I in Drosophila species and hybrids: The co-evolution of an enhancer and its cognate factor
| Autor: | Krzysztof Jagla, Philippe Georgel, Guy Dretzen, François Bellard, Maria Bellard, Geoff Richards |
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| Rok vydání: | 1992 |
| Předmět: |
Male
Molecular Sequence Data Enhancer binding Drosophilidae Genetics Melanogaster Animals Enhancer Molecular Biology Gene Crosses Genetic Base Sequence biology Glue Proteins Drosophila DNA Blotting Northern biology.organism_classification Biological Evolution Chromatin Drosophila melanogaster Enhancer Elements Genetic Regulatory sequence Drosophila Female Protein Binding |
| Zdroj: | Molecular and General Genetics MGG. 235:104-112 |
| ISSN: | 1432-1874 0026-8925 |
| DOI: | 10.1007/bf00286187 |
| Popis: | The activation of the Drosophila melanogaster salivary gland secretion protein gene Sgs-3 is marked by important changes in chromatin structure in the distal regulatory region at − 600 by from the Sgs-3 start site. A stage- and tissue-specific glue enhancer binding factor, GEBF-I, binds in vitro to sequences from this region. Previous studies have revealed considerable variation in the DNA sequences of comparable regions in the related Drosophila species, D. simulans, D. erecta and D. yakuba. We detected GEBF-I-like proteins in these species, which appear to evolve as rapidly as the corresponding DNA sequences, and studied in detail the binding characteristics of the GEBF-I proteins of the two most closely related species, D. melanogaster and D. simulans. In crosses between these species, certain strains produce hybrid larvae which, unexpectedly, synthesised a single intermediate form of the protein. This suggests that the factor is subject to species-specific post-transcriptional modifications. In these hybrid larvae, which carry one D. melanogaster and one D. simulans Sgs-3 gene, the hybrid GEBF-I protein appears equally effective in the induction of both target genes. |
| Databáze: | OpenAIRE |
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