Immunoaffinity chromatography of bovine FSH using monoclonal antibodies
| Autor: | R.A. Goldsby, D.J. Bolt, K. F. Miller |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
biology
Chemistry medicine.drug_class Endocrinology Diabetes and Metabolism Antibodies Monoclonal Spleen Immunoglobulin light chain Monoclonal antibody Molecular biology Chromatography Affinity Dissociation constant Endocrinology medicine.anatomical_structure Affinity chromatography Anterior pituitary Cell culture medicine biology.protein Animals Cattle Follicle Stimulating Hormone Antibody |
| Zdroj: | Journal of Endocrinology. 115:283-288 |
| ISSN: | 1479-6805 0022-0795 |
| DOI: | 10.1677/joe.0.1150283 |
| Popis: | Bovine FSH (bFSH) was used to immunize BALB/c mice. Spleen cells were fused to the SP 2/0 cell line to produce hybridomas that secreted monoclonal antibodies to bFSH. One of these antibodies (USDA-bFSH-MC28) was extensively characterized and found to be a gamma 1 with kappa light chains, having extremely low cross-reactivity with other bovine pituitary hormones and with ovine and porcine FSH. The dissociation constant as measured by Scatchard analysis was 4·3 nmol/l, and proved to be in a very useful range for affinity chromatography. In an essentially one-step immunoaffinity chromatography procedure, bFSH was easily isolated in a single chromatographic step from crude anterior pituitary homogenate with better yield and with the same purity as classical chromatographic techniques. J. Endocr. (1987) 115, 283–288 |
| Databáze: | OpenAIRE |
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