Purification, crystallization and preliminary crystallographic analysis of peroxidase from the palm tree Chamaerops excelsa

Autor: Jademilson C. Santos, Igor Polikarpov, Larissa C. Textor, Galina G. Zhadan, Valery L. Shnyrov, Manuel G. Roig, Nazaret Hidalgo Cuadrado
Rok vydání: 2011
Předmět:
Zdroj: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
ISSN: 1744-3091
Popis: Plant peroxidases are presently used extensively in a wide range of biotechnological applications owing to their high environmental and thermal stability. As part of efforts towards the discovery of appealing new biotechnological enzymes, the peroxidase from leaves of the palm tree Chamaerops excelsa (CEP) was extracted, purified and crystallized in its native form. An X-ray diffraction data set was collected at a synchrotron source and data analysis showed that the CEP crystals belonged to the orthorhombic space group P212121, with unit-cell parameters a = 70.2, b = 100.7, c = 132.3 Å.
Databáze: OpenAIRE