Purification, crystallization and preliminary crystallographic analysis of peroxidase from the palm tree Chamaerops excelsa
| Autor: | Jademilson C. Santos, Igor Polikarpov, Larissa C. Textor, Galina G. Zhadan, Valery L. Shnyrov, Manuel G. Roig, Nazaret Hidalgo Cuadrado |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
biology
Chemistry Biophysics Arecaceae Crystallography X-Ray Condensed Matter Physics biology.organism_classification Biochemistry law.invention Crystallography Tree (descriptive set theory) Peroxidases Chamaerops Crystallization Communications Structural Biology law PALMEIRAS Genetics biology.protein Orthorhombic crystal system Crystallization Palm Peroxidase |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1744-3091 |
| Popis: | Plant peroxidases are presently used extensively in a wide range of biotechnological applications owing to their high environmental and thermal stability. As part of efforts towards the discovery of appealing new biotechnological enzymes, the peroxidase from leaves of the palm tree Chamaerops excelsa (CEP) was extracted, purified and crystallized in its native form. An X-ray diffraction data set was collected at a synchrotron source and data analysis showed that the CEP crystals belonged to the orthorhombic space group P212121, with unit-cell parameters a = 70.2, b = 100.7, c = 132.3 Å. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text