Evaluation of the ability of bone marrow derived cells to engraft the kidney and promote renal tubular regeneration in mice following exposure to cisplatin

Autor: Eric Rondeau, Pierre Galichon, Aurélien Bataille, Alexandre Hertig, David Legouis, Sophie Vandermeersch, Morgane Wetzstein
Přispěvatelé: Des Maladies Rénales Rares aux Maladies Fréquentes, Remodelage et Réparation, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Pathology
medicine.medical_specialty
Kidney/physiology
Kidney Tubules/physiology
Antineoplastic Agents
Bone Marrow Cells
Stem cells
Critical Care and Intensive Care Medicine
Kidney
Cell therapy
03 medical and health sciences
Mice
Acute Kidney Injury/chemically induced/surgery
medicine
Animals
Regeneration
Renal stem cell
Bone Marrow Transplantation
Cisplatin
business.industry
Acute kidney injury
General Medicine
Antineoplastic Agents/adverse effects
Acute Kidney Injury
medicine.disease
Bone Marrow Cells/physiology
3. Good health
Mice
Inbred C57BL

030104 developmental biology
medicine.anatomical_structure
Kidney Tubules
Nephrology
Apoptosis
Cisplatin/adverse effects
Female
Bone marrow
Stem cell
business
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
medicine.drug
Zdroj: Renal Failure
Renal Failure, Taylor & Francis, 2016, 38 (4), pp.521-529. ⟨10.3109/0886022X.2016.1145521⟩
Renal Failure, 2016, 38 (4), pp.521-529. ⟨10.3109/0886022X.2016.1145521⟩
Renal Failure, Vol. 38, No 4 (2016) pp. 521-529
ISSN: 0886-022X
1525-6049
DOI: 10.3109/0886022X.2016.1145521⟩
Popis: International audience; It has been suggested that bone marrow derived stem cells have the ability to engraft the kidney and improve the outcome of severe acute kidney injury (AKI) in mice exposed to high doses of cisplatin, providing hope for cancer patients in whom irreversible renal damage occasionally occurs following the use of this highly effective anti-tumor drug. We tested the therapeutic potential of bone marrow derived cells injected during the acute phase (day 3 after cisplatin administration) of experimentally-induced AKI in C57Bl6/J mice, characterized by massive tubular necrosis, apoptosis, and a low proliferation capacity. We failed to show any benefit of bone marrow derived cells versus a regular homogenate of intact renal cells, or normal saline. Using cell tracers and flow cytometry, we demonstrated that bone marrow derived cells did indeed home to the bone marrow of the recipients but failed to settle in the kidney. Conversely, renal cells homed to injured kidneys. However, neither cell therapy protected the animals against cisplatin-induced death. We therefore question the short-term efficacy of bone marrow derived cells used to repair established injuries of the tubular epithelium.
Databáze: OpenAIRE