Evaluation of the ability of bone marrow derived cells to engraft the kidney and promote renal tubular regeneration in mice following exposure to cisplatin
Autor: | Eric Rondeau, Pierre Galichon, Aurélien Bataille, Alexandre Hertig, David Legouis, Sophie Vandermeersch, Morgane Wetzstein |
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Přispěvatelé: | Des Maladies Rénales Rares aux Maladies Fréquentes, Remodelage et Réparation, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Kidney/physiology Kidney Tubules/physiology Antineoplastic Agents Bone Marrow Cells Stem cells Critical Care and Intensive Care Medicine Kidney Cell therapy 03 medical and health sciences Mice Acute Kidney Injury/chemically induced/surgery medicine Animals Regeneration Renal stem cell Bone Marrow Transplantation Cisplatin business.industry Acute kidney injury General Medicine Antineoplastic Agents/adverse effects Acute Kidney Injury medicine.disease Bone Marrow Cells/physiology 3. Good health Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Kidney Tubules Nephrology Apoptosis Cisplatin/adverse effects Female Bone marrow Stem cell business [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology medicine.drug |
Zdroj: | Renal Failure Renal Failure, Taylor & Francis, 2016, 38 (4), pp.521-529. ⟨10.3109/0886022X.2016.1145521⟩ Renal Failure, 2016, 38 (4), pp.521-529. ⟨10.3109/0886022X.2016.1145521⟩ Renal Failure, Vol. 38, No 4 (2016) pp. 521-529 |
ISSN: | 0886-022X 1525-6049 |
DOI: | 10.3109/0886022X.2016.1145521⟩ |
Popis: | International audience; It has been suggested that bone marrow derived stem cells have the ability to engraft the kidney and improve the outcome of severe acute kidney injury (AKI) in mice exposed to high doses of cisplatin, providing hope for cancer patients in whom irreversible renal damage occasionally occurs following the use of this highly effective anti-tumor drug. We tested the therapeutic potential of bone marrow derived cells injected during the acute phase (day 3 after cisplatin administration) of experimentally-induced AKI in C57Bl6/J mice, characterized by massive tubular necrosis, apoptosis, and a low proliferation capacity. We failed to show any benefit of bone marrow derived cells versus a regular homogenate of intact renal cells, or normal saline. Using cell tracers and flow cytometry, we demonstrated that bone marrow derived cells did indeed home to the bone marrow of the recipients but failed to settle in the kidney. Conversely, renal cells homed to injured kidneys. However, neither cell therapy protected the animals against cisplatin-induced death. We therefore question the short-term efficacy of bone marrow derived cells used to repair established injuries of the tubular epithelium. |
Databáze: | OpenAIRE |
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