Expression of cloned aromatic L-amino acid decarboxylase in Xenopus laevis oocytes
| Autor: | Norton H. Neff, K.P. Gudehithlu, Anne-Marie Duchemin, C. P. Silvia, Maria Hadjiconstantinou |
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| Rok vydání: | 1992 |
| Předmět: |
Microinjections
Transcription Genetic Xenopus Cellular and Molecular Neuroscience Xenopus laevis Gene expression Adrenal Glands medicine Animals RNA Messenger Cloning Molecular Microinjection Gene Library chemistry.chemical_classification Messenger RNA Aromatic L-amino acid decarboxylase biology Cell Biology DNA biology.organism_classification Molecular biology Enzyme assay Kinetics medicine.anatomical_structure Enzyme Biochemistry chemistry Aromatic-L-Amino-Acid Decarboxylases Protein Biosynthesis biology.protein Oocytes Cattle Female Adrenal medulla |
| Zdroj: | Neurochemistry international. 21(2) |
| ISSN: | 0197-0186 |
| Popis: | Sense mRNA coding for bovine adrenal medulla aromatic l -amino acid decarboxylase (AADC) was expressed following microinjection into Xenopus laevis oocytes. The expressed enzyme activity was stereoselective for l -5-hydroxytryptophan and l -DOPA and blocked by NSD-1015 an inhibitor of AADC. Heating the expressed enzyme at 55°C resulted in a parallel loss of activity towards both substrates. Our findings are consistent with the prevailing notion that a single enzyme is able to decarboxylate both substrates in vivo . |
| Databáze: | OpenAIRE |
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