Genetic characterization of Chikungunya virus 2009 isolates from South India

Autor:	Padma Srikanth, Sathish Sankar, Seema A. Nayar, Sudharsana Sundarrajan, Gracy Fathima Selvaraj, Gunasekaran Palani, J. Damodharan, Gopalsamy Sarangan, Karuppaiah Muthumani, Monika Mani
Rok vydání:	2018
Předmět:	Genetic diversity Lineage (genetic) Phylogenetic tree biology RT-PCR virus diseases Outbreak partial E2 gene RNA virus General Medicine Hypothesis medicine.disease_cause biology.organism_classification substitutions Virology Virus selection pressure Genotype medicine Chikungunya Chikungunya virus
Zdroj:	Bioinformation
ISSN:	0973-2063 0973-8894
DOI:	10.6026/97320630014106
Popis:	Chikungunya Virus (CHIKV) is a single stranded positive sense enveloped RNA virus. Re-emergence of CHIKV caused a massive outbreak with severe clinical manifestation affecting multiple organs. The genetic diversity of CHIKV, which caused recurring outbreaks in India, was studied. Blood samples were collected from suspected human cases of CHIKV infection in Chennai, Tamil Nadu and three Northern districts of Kerala in Southern India during the CHIKV outbreak in 2009. A partial E2 gene segment was amplified by RT-PCR. Among 119 samples 37 samples were positive for CHIKV by RT-PCR. Phylogenetic analysis revealed that the isolated sequences belonged to Indian Ocean Lineage (IOL) of ECSA genotype. The mutational analysis revealed the presence of substitutions such as S299N, T312M, A344T, S375T, V386G, W339R and S375P in the current study. In addition, a novel mutation V386G was observed in all the sequences. Two isolates found with unique substitutions W339R and S375P are reported. The structural analysis of the wild type and mutant proteins revealed that the structural changes are accompanied by modification in the intraprotein interactions.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa4cf9b858975c8fbcb0cb359adb8494 https://doi.org/10.6026/97320630014106 Zobrazit plný text záznamu