Prevalence and phylogenetic analysis of haemoplasmas from cats infected with multiple species
| Autor: | Larissa C. Aquino, Chelsea A E Hicks, Marcelle Santos Lemos, Séverine Tasker, Marcela C. Scalon, Giane Regina Paludo, Christopher R Helps, Maíra Gonçalves da Mota Lima |
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| Jazyk: | angličtina |
| Předmět: |
Microbiology (medical)
medicine.disease_cause Cat Diseases Microbiology Article Mycoplasma Phylogenetics RNA Ribosomal 16S medicine Prevalence Animals Mycoplasma Infections Molecular Biology Phylogeny Genetics CATS biology Phylogenetic tree Co-infections Biodiversity Ribosomal RNA biology.organism_classification 16S ribosomal RNA Virology Haemoplasmas Mycoplasma haemofelis GenBank Cats Brazil |
| Zdroj: | Journal of Microbiological Methods Aquino, L C, Hicks, C A E, Scalon, M C, Lima, M G D M, Lemos, M D S, Paludo, G R, Helps, C R & Tasker, S 2014, ' Prevalence and phylogenetic analysis of haemoplasmas from cats infected with multiple species ', Journal of Microbiological Methods, vol. 107, pp. 189-196 . https://doi.org/10.1016/j.mimet.2014.10.013 |
| ISSN: | 0167-7012 |
| DOI: | 10.1016/j.mimet.2014.10.013 |
| Popis: | Mycoplasma haemofelis (Mhf), ‘Candidatus Mycoplasma haemominutum’ (CMhm) and ‘Candidatus Mycoplasma turicensis’ (CMt) are agents of feline haemoplasmosis and can induce anaemia in cats. This study aimed to determine the prevalence and phylogeny of haemoplasma species in cats from Brazil's capital and surrounding areas, and whether correlation with haematological abnormalities existed. Feline haemoplasmas were found in 13.8% of 432 cats. CMhm was the most prevalent species (in 13.8% of cats), followed by Mhf (11.1%) and CMt (4.4%). Over 80% of haemoplasma-infected cats harboured two or more feline haemoplasma species: 7.1% of cats were co-infected with Mhf/CMhm, 0.4% with CMhm/CMt and 3.9% with Mhf/CMhm/CMt. Male gender was significantly associated with haemoplasma infections. No association was found between qPCR haemoplasma status and haematological variables, however CMhm relative copy numbers were correlated with red blood cell (RBC) numbers and packed cell volume (PCV). Haemoplasma 16S rRNA gene sequences (> 1 Kb) were derived from co-infected cats using novel haemoplasma species-specific primers. This allowed 16S rRNA gene sequences to be obtained despite the high level of co-infection, which precluded the use of universal 16S rRNA gene primers. Within each species, the Mhf, CMhm and CMt sequences showed > 99.8%, > 98.5% and > 98.8% identity, respectively. The Mhf, CMhm and CMt sequences showed > 99.2%, > 98.4% and > 97.8% identity, respectively, with GenBank sequences. Phylogenetic analysis showed all Mhf sequences to reside in a single clade, whereas the CMhm and CMt sequences each grouped into three distinct subclades. These phylogeny findings suggest the existence of different CMhm and CMt strains. Highlights • Over 80% of haemoplasma-infected cats had more than one haemoplasma species. • The use of species-specific primers allowed derivation of 16S rDNA sequences from co-infected cats. • Male gender was significantly associated with haemoplasma infection. • Phylogenetic analysis showed subclade formation within two of the haemoplasma species sequences. |
| Databáze: | OpenAIRE |
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