Dietary exposure to benzoxazinoids enhances bacteria-induced monokine responses by peripheral blood mononuclear cells
| Autor: | Yaseelan Palarasah, Michael Friberg Bruun Nielsen, Claus Henrik Nielsen, Lars K. Poulsen, Heidi J. Schnoor, Khem B. Adhikari, Inge S. Fomsgaard, Nanna Juel-Berg, Dres Damgaard, Bettina M. Jensen |
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| Rok vydání: | 2015 |
| Předmět: |
Lipopolysaccharides
Lipopolysaccharide T-Lymphocytes medicine.medical_treatment Inflammation Stimulation Peripheral blood mononuclear cell chemistry.chemical_compound Tetanus Toxoid/pharmacology Tetanus Toxoid Humans Medicine Leukocytes Mononuclear/immunology Benzoxazines/administration & dosage Porphyromonas gingivalis Cross-Over Studies biology business.industry Monokines lipopolysaccharide Toxoid benzoxazinoids T-Lymphocytes/immunology Lipopolysaccharides/pharmacology biology.organism_classification Benzoxazines Blood Cell Count Diet Monokine Monokines/biosynthesis Cytokine chemistry inflammation Immunology monokines Leukocytes Mononuclear medicine.symptom business Food Science Biotechnology |
| Zdroj: | Damgaard, D, Jensen, B M, Palarasah, Y, Nielsen, M F B, Adhikari, K B, Schnoor, H J, Juel-Berg, N, Poulsen, L K, Fomsgaard, I S & Nielsen, C H 2015, ' Dietary exposure to benzoxazinoids enhances bacteria-induced monokine responses by peripheral blood mononuclear cells ', Molecular Nutrition & Food Research, vol. 59, no. 11, pp. 2190-2198 . https://doi.org/10.1002/mnfr.201500151 |
| ISSN: | 1613-4125 |
| DOI: | 10.1002/mnfr.201500151 |
| Popis: | SCOPE: To examine potentially immunomodulating effects of dietary benzoxazinoids (BXs), present in cereal grains.METHODS AND RESULTS: Nineteen healthy volunteers were randomly distributed into two groups, who received diets with high or low content of BXs for three weeks. After a week's wash-out, the groups switched diets. Peripheral blood mononuclear cells (PBMCs) were stimulated with Porphyromonas gingivalis, Escherichia coli lipopolysaccharide (LPS), or tetanus toxoid (TT). PBMCs from a healthy donor received the same stimuli in presence of serum from each participant receiving BXs. The production of monokines, T-cell cytokines and T-helper cell proliferation were assessed. A three-week diet with high BX content enhanced IL-1β responses against LPS and P. gingivalis, as well as TNF-α response against P. gingivalis, after 24 hours of stimulation. Moreover, IL-6 was found to be increased after 7 days of stimulation with LPS. No effect was observed on T-cell cytokines or proliferation. BX levels in serum after a single meal did not modify cytokine responses.CONCLUSIONS: High dietary intake of BXs enhances bacteria-induced production of pro-inflammatory monokines by PBMCs, but not T-cell responses; presumably due to intrinsic changes within PBMCs, built up over three weeks of BX-rich diet, rather than to immediate effects of BXs contained in serum. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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