Effect of perfluorooctane sulfonate on viability, maturation and gap junctional intercellular communication of porcine oocytes in vitro
Autor: | E Arenas, Miguel Betancourt, Yvonne Ducolomb, Edmundo Bonilla, A. Domínguez, Zayil Salazar, Humberto González-Márquez, M. Teteltitla, Eduardo Casas |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
medicine.medical_specialty Cell signaling Cell Survival Swine Gene Expression Perfluorinated compound Connexin Cell Communication Biology Toxicology Connexins Andrology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Animals Cells Cultured Fluorocarbons Granulosa Cells 030219 obstetrics & reproductive medicine Gap junction Gap Junctions General Medicine Oocyte Follicular fluid In vitro Perfluorooctane 030104 developmental biology medicine.anatomical_structure Endocrinology Alkanesulfonic Acids chemistry Oocytes Female |
Zdroj: | Toxicology in Vitro. 35:93-99 |
ISSN: | 0887-2333 |
Popis: | Perfluorooctane sulfonate (PFOS) is a broadly used man-made surfactant whose long half-life has led to bioaccumulation. This perfluorinated compound is ubiquitous in human body fluids. PFOS concentrations as high as 26μM in plasma have been reported in occupationally exposed populations, and high levels of PFOS in human follicular fluid have been associated with subfertility. However, the effect of PFOS on the maturation of oocytes in mammals has not been reported to date. The aim of this study was to determine the effects of PFOS during oocyte maturation. Results indicate that PFOS inhibits oocyte viability (Lethal Concentration50=32μM) and maturation (inhibition of maturation50=22μM) at physiologically relevant concentrations. In order to evaluate the mechanisms of oocyte maturation inhibition by PFOS, gap junctional intercellular communication (GJIC) between oocytes and granulosa cells was assessed. GJIC between granulosa cells and the oocyte was significantly affected during the first 8h of maturation. However, the inhibitory effect of PFOS on GJIC was not due to an alteration on the expression of connexin genes Cx43, Cx45 and Cx60. These findings suggest that occupationally exposed populations could be at risk, and that PFOS might affect oocyte maturation by interfering the GJIC in the cumulus-oocyte complexes during the first hours of maturation. |
Databáze: | OpenAIRE |
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