Transcription of the human cell cycle regulated BUB1B gene requires hStaf/ZNF143
| Autor: | Alain Krol, Evelyne Myslinski, Philippe Carbon, Marie-Aline Gérard |
|---|---|
| Přispěvatelé: | Architecture et réactivité de l'ARN (ARN), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2007 |
| Předmět: |
MESH: Drosophila
5' Flanking Region Response element MESH: Cell Cycle MESH: Base Sequence 0302 clinical medicine Chlorocebus aethiops E2F1 MESH: Animals Promoter Regions Genetic 0303 health sciences Cell Cycle TCF4 Cell Cycle Gene MESH: COS Cells DNA-Binding Proteins MESH: Promoter Regions (Genetics) MESH: DNA Transposable Elements 030220 oncology & carcinogenesis COS Cells TAF2 Drosophila MESH: Transcription Initiation Site Transcription Initiation Site Transcriptional Activation MESH: Mutation MESH: Trans-Activators Molecular Sequence Data Protein Serine-Threonine Kinases Biology Cell Line 03 medical and health sciences Sp3 transcription factor Genetics Animals Humans [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Enhancer MESH: Protein Kinases Molecular Biology 030304 developmental biology MESH: 5' Flanking Region MESH: Humans MESH: Molecular Sequence Data Binding Sites Base Sequence Promoter MESH: Cercopithecus aethiops Molecular biology MESH: Cell Line MESH: Binding Sites MESH: Trans-Activation (Genetics) Mutation DNA Transposable Elements Trans-Activators Protein Kinases MESH: DNA-Binding Proteins |
| Zdroj: | Nucleic Acids Research Nucleic Acids Research, Oxford University Press, 2007, 35 (10), pp.3453-64. ⟨10.1093/nar/gkm239⟩ |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gkm239 |
| Popis: | International audience; BubR1 is a key protein mediating spindle checkpoint activation. Loss of this checkpoint control results in chromosomal instability and aneuploidy. The transcriptional regulation of the cell cycle regulated human BUB1B gene, which encodes BubR1, was investigated in this report. A minimal BUB1B gene promoter containing 464 bp upstream from the translation initiation codon was sufficient for cell cycle regulated promoter activity. A pivotal role for transcription factor hStaf/ZNF143 in the expression of the BUB1B gene was demonstrated through gel retardation assays, transient expression of mutant BUB1B promoter-reporter gene constructs and chromatin immunoprecipitation assay. Two phylogenetically conserved hStaf/ZNF143-binding sites (SBS) were identified which are indispensable for BUB1B promoter activity. In addition, we found that the domain covering the transcription start sites contains conserved boxes homologous to initiator (Inr), cell cycle dependent (CDE) and cell cycle genes homology regions (CHR) elements. Mutations within the CDE and CHR elements led to diminished cell cycle regulation of BUB1B transcription. These results demonstrate that BUB1B gene transcription is positively regulated by hStaf/ZNF143, a ubiquitously expressed factor, and that the CDE-CHR tandem element was essential for G2/M-specific transcription of the BUB1B gene. |
| Databáze: | OpenAIRE |
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