Effect of cryoprotectants on the survival of cascudo preto (Rhinelepis aspera) embryos stored at –8°C
| Autor: | Danilo Pedro Streit, Rodolfo Nardez Sirol, Leandro Godoy, Diego de Oliveira, Patrícia Ribeiro Neves, Darci Carlos Fornari, Ricardo Pereira Ribeiro |
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| Rok vydání: | 2011 |
| Předmět: |
Ethylene Glycol
Sucrose Embryo Nonmammalian Cryobiology Cryoprotectant Embryonic Development Aquaculture Cryopreservation chemistry.chemical_compound Cryoprotective Agents Animal science Animals Catfishes biology Hatching Methanol Rhinelepis aspera Cell Biology biology.organism_classification chemistry Biochemistry Ethylene glycol Brazil Developmental Biology |
| Zdroj: | Zygote. 22:58-63 |
| ISSN: | 1469-8730 0967-1994 |
| DOI: | 10.1017/s0967199411000517 |
| Popis: | SummaryCryopreservation of germplasm provides a promising method to preserve fish genetic material, which is of great importance in preservation of species diversity, aquaculture, and management of fish models used in biomedical research. In the present study, cryopreservation of Rhinelepis aspera embryos, a Brazilian endangered species, was studied for the first time using a short-term cooling protocol. Embryos at blastoporous closing stage were selected, placed in 6-ml glass vials and stored at −8°C for 6 h in 10 different cryoprotectant solutions: S1 (17.1% sucrose + 9% methanol); S2 (17.1% sucrose + 9% DMSO); S3 (8.5% sucrose + 8.5% glucose + 9% methanol); S4 (8.5% sucrose + 8.5% glucose + 9% DMSO); S5 (17.1% sucrose + 9% ethylene glycol); S6 (8.5% sucrose + 8.5% glucose + 9% ethylene glycol); S7 (17.1% sucrose + 4.5% methanol + 4.5% DMSO); S8 (17.1% sucrose + 4.5% methanol + 4.5% ethylene glycol); S9 (17.1% sucrose + 4.5% DMSO + 4.5% ethylene glycol); and S10 (100% water). Embryo viability was assessed by hatching rate, counting live larvae and number of failed eggs under a stereomicroscope. The results showed that only the cryoprotectant solutions that contained methanol associated to sucrose (S1, S7 and S8) provided partial protection of Rhinelepis aspera embryos from cold damage (over 50% hatching rate in S1), while the use of DMSO and ethylene glycol, isolated or in combination, resulted in no hatching rate. Further studies are needed in order to extend the storage time and to improve the hatching rate for the species. |
| Databáze: | OpenAIRE |
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