Enhanced Goblet Cell Hyperplasia in HDC Knockout Mice with Allergic Airway Inflammation
| Autor: | Harumasa Ito, Takashi Mouri, Hiroshi Ohtsu, Hong Mei Piao, Yutaka Nakamura, Toshihide Nakadate, Hitoshi Kobayashi, Hiroshi Inoue, Takashi Sawai, Kohei Yamauchi, Toshiki Shikanai, Chihaya Maesawa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
Histidine Decarboxylase Mucin 5AC Immunoglobulin E chemistry.chemical_compound Histamine receptor Mice airway remodeling Mucoproteins Gob-5 Chloride Channels medicine Immunology and Allergy Animals Mice Knockout Hyperplasia biology Chemistry Cell Differentiation General Medicine respiratory system asthma medicine.disease MUC5AC Histidine decarboxylase Mice Inbred C57BL Ovalbumin TNF-α Immunology biology.protein Respiratory epithelium Cytokines Tumor necrosis factor alpha Female Goblet Cells lcsh:RC581-607 Bronchoalveolar Lavage Fluid Histamine |
| Zdroj: | Allergology International, Vol 58, Iss 1, Pp 125-134 (2009) |
| ISSN: | 1323-8930 |
| Popis: | Background Histamine is known to have immunoregulatory roles in allergic reactions through histamine receptor 1 (H1R), H2R, H3R and H4R. However, its role in goblet cell hyperplasia in the airways of asthma patients is yet to be clarified. Objective This study was designed to examine the role of histamine in goblet cell hyperplasia using histamine-deficient mice ( Hdc -/- mice) with allergic airway inflammation. Methods Wild-type and Hdc -/- C57BL/6 mice were sensitized with ovalbumin (OVA). After a 2-week exposure to OVA, goblet cell hyperplasia was evaluated. Cell differentials and cytokines in BALF were analyzed. The mRNA levels of MUC5AC and Gob-5 gene were determined quantitatively. Results The number of eosinophils in BALF increased in both the sensitized wild-type mice and Hdc -/- mice with OVA inhalation. In addition, the numbers of alveolar macrophages and lymphocytes in BALF increased significantly in the sensitized Hdc -/- mice with OVA inhalation compared to the wild-type mice under the same conditions. The concentrations of Interleukin-4 (IL-4), IL-5, IL-13, Interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α) and IL-2 in the BALF all increased significantly in both groups compared to those exposed to saline. In particular, the concentration of TNF-α in the Hdc -/- mice exposed to OVA was significantly higher than that in the wild-type mice under the same conditions. The mRNA levels of Gob-5 and MUC5AC, and the ratio of the goblet cells in the airway epithelium significantly increased in Hdc -/- mice exposed to OVA compared to wild-type mice. Conclusions These results suggested that histamine may play a regulatory role in goblet cell hyperplasia in allergic airway inflammation. |
| Databáze: | OpenAIRE |
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