Transcriptomes of antigen presenting cells in human thymus
| Autor: | Isaac Snowhite, H E Akselsen, Hanna Helgeland, Arvind Y. M. Sundaram, Siri Tennebø Flåm, Benedicte A. Lie, Ingvild Synnøve Matre Gabrielsen, Hans Christian Dalsbotten Aass, Alberto Pugliese |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine B Cells Molecular biology Thrombomodulin Gene Expression Transcriptome White Blood Cells Sequencing techniques 0302 clinical medicine HLA Antigens Animal Cells Medicine and Health Sciences Antigen Presentation B-Lymphocytes Multidisciplinary biology Transcriptional Control RNA sequencing Thymus Cell biology DNA-Binding Proteins Antigens Surface Medicine Cellular Types Central tolerance Research Article Immune Cells Science Primary Cell Culture Immunology Interleukin-3 Receptor alpha Subunit Antigen-Presenting Cells Thymus Gland Human leukocyte antigen CD19 Immunophenotyping Autoimmune Diseases 03 medical and health sciences Antigen Gene Types Genetics Humans Gene Regulation Antibody-Producing Cells Antigen-presenting cell Gene Blood Cells Gene Expression Profiling Infant Newborn Infant Biology and Life Sciences Epithelial Cells Dendritic Cells Cell Biology Research and analysis methods Molecular biology techniques 030104 developmental biology Cell culture Immune System biology.protein Regulator Genes Clinical Immunology Clinical Medicine Transcription Factors 030215 immunology |
| Zdroj: | PLoS ONE, Vol 14, Iss 7, p e0218858 (2019) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0218858 |
| Popis: | Antigen presenting cells (APCs) in the thymus play an essential role in the establishment of central tolerance, i.e. the generation of a repertoire of functional and self-tolerant T cells to prevent autoimmunity. In this study, we have compared the transcriptomes of four primary APCs from human thymus (mTECs, CD19+ B cells, CD141+ and CD123+ DCs). We investigated a set of genes including the HLA genes, genes encoding transcriptional regulators and finally, tissue-enriched genes, i.e, genes with a five-fold higher expression in a particular human tissue. We show that thymic CD141+ DCs express the highest levels of all classical HLA genes and 67% (14/21) of the HLA class I and II pathway genes investigated in this study. CD141+ DCs also expressed the highest levels of the transcriptional regulator DEAF1, whereas AIRE and FEZF2 expression were mainly found in primary human mTECs. We found expression of “tissue enriched genes” from the Human Protein Atlas (HPA) in all four APC types, but the mTECs were clearly dominating in the number of uniquely expressed tissue enriched genes (20% in mTECs, 7% in CD19+ B cells, 4% in CD123+ DCs and 2% in CD141+ DCs). The tissue enriched genes also overlapped with reported human autoantigens. This is, to our knowledge, the first study that performs RNA sequencing of mTECs, CD19+ B cells, CD141+ and CD123+ DCs isolated from the same individuals and provides insight into the transcriptomes of these human thymic APCs. |
| Databáze: | OpenAIRE |
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