Prostate-specific membrane antigen can promote in vivo osseous metastasis of prostate cancer cells in mice

Autor: Shengjie Guo, Kai-Yuan Chao, Shaopeng Qiu, Liang-yun Zhao, Xiaopeng Mao
Rok vydání: 2012
Předmět:
Glutamate Carboxypeptidase II
Male
Vascular Endothelial Growth Factor A
Pathology
Bone density
Physiology
Prostate-specific membrane antigen
Lytic Bone Lesion
urologic and male genital diseases
Bone tissue
Biochemistry
Mice
Prostate cancer
chemistry.chemical_compound
Bone Density
Medicine
General Pharmacology
Toxicology and Pharmaceutics
lcsh:QH301-705.5
lcsh:R5-920
General Neuroscience
General Medicine
Immunohistochemistry
Vascular endothelial growth factor
Vascular endothelial growth factor A
medicine.anatomical_structure
Matrix Metalloproteinase 9
Antigens
Surface
Female
Osseous metastasis
lcsh:Medicine (General)
medicine.medical_specialty
Short Communication
Immunology
Biophysics
Bone Neoplasms
Prostate carcinoma
In vitro
In vivo
Cell Line
Tumor
Animals
business.industry
Prostatic Neoplasms
Neoplasms
Experimental
Cell Biology
medicine.disease
Mice
Inbred C57BL
lcsh:Biology (General)
chemistry
business
Zdroj: Brazilian Journal of Medical and Biological Research, Vol 45, Iss 8, Pp 737-745 (2012)
Brazilian Journal of Medical and Biological Research v.45 n.8 2012
Brazilian Journal of Medical and Biological Research
Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
Brazilian Journal of Medical and Biological Research, Volume: 45, Issue: 8, Pages: 737-745, Published: AUG 2012
ISSN: 0100-879X
DOI: 10.1590/s0100-879x2012007500085
Popis: Reports remain insufficient on whether and how prostate-specific membrane antigen (PSMA) can influence in vivo osseous metastasis of prostate cancer (PCa). In the present study, the authors induced stable expression of PSMA in mouse PCa cell line RM-1. In vivo osseous metastasis was induced in 37 6-week-old female C57BL/6 mice weighing 22.45 ± 0.456 g. RM-1 cells were actively injected into the femoral bone cavity, leading to bilateral dissymmetry of bone density in the femoral bone. Tumor cells were also detected in bone tissue by pathological examination. The impact on bone density was demonstrated by the significant difference between animals injected with RM-PSMA cells (0.0738 ± 0.0185 g/cm²) and animals injected with RM-empty plasmid cells (0.0895 ± 0.0241 g/cm²). The lytic bone lesion of the RM-PSMA group (68.4%) was higher than that of the control group (27.8%). Immunohistochemistry showed that the expression of both vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) was distinctly higher in the RM-PSMA group than in the control group, while ELISA and Western blot assay indicated that VEGF and MMP-9 were higher in the RM-PSMA group compared to the control group (in vitro). Thus, the present study proposed and then confirmed for the first time that PSMA can promote in vivo osseous metastasis of PCa by increasing sclerotic destruction of PCa cells. Further analyses also suggested that PSMA functions positively on the invasive ability of RM-1 by increasing the expression of MMP-9 and VEGF by osseous metastases in vivo.
Databáze: OpenAIRE
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