Two highly related homeodomain proteins, Nkx5-1 and Nkx5-2, display different DNA binding specificities
| Autor: | Eva Bober, Sonja Hoffmann, Hans-Henning Arnold, Thorsten Hadrys, Detlev Mennerich |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Genetics
Homeodomain Proteins Binding Sites biology HMG-box Sequence Homology Amino Acid Recombinant Fusion Proteins Clinical Biochemistry Molecular Sequence Data Nerve Tissue Proteins DNA Biochemistry Single-stranded binding protein DNA binding site Mice biology.protein Homeobox Animals Amino Acid Sequence Binding site Homologous recombination Molecular Biology Gene Binding domain Glutathione Transferase |
| Zdroj: | Biological chemistry. 380(9) |
| ISSN: | 1431-6730 |
| Popis: | The mouse Nkx5-1 and Nkx5-2 genes are related to NK genes in Drosophila and encode proteins with very similar homeodomains. In higher vertebrates Nkx5 genes are specifically expressed in the inner ear. Inactivation of the mouse Nkx5-1 gene by homologous recombination revealed a critical role for the formation of vestibular inner ear structures. Here, we investigated biochemical properties of the proteins encoded by the Nkx5 genes. A similar consensus binding sequence was isolated for both Nkx5 proteins using binding site selection. This sequence is related to target sequences previously identified for other Nkx proteins and contains the conserved homeodomain binding core TAAT. An additional, novel and unrelated high affinity binding sequence could be identified for the Nkx5-2 protein. |
| Databáze: | OpenAIRE |
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